Angiogenesis in patients with craniopharyngiomas

Correlation with treatment and outcome

Sergio Vidal, Kalman Kovacs, Ricardo V. Lloyd, Frederic B. Meyer, Bernd W. Scheithauer

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

BACKGROUND. Craniopharyngiomas are histologically benign epithelial neoplasms of the sellar region that often exhibit aggressive and invasive growth. The authors hypothesized that tumor proliferation, spread, and recurrence are angiogenesis dependent and investigated the significance of vascularization relative to biologic behavior. To the authors' knowledge, angiogenesis for patients with craniopharyngiomas has not been examined to date. METHODS. The authors measured microvessel densities in resected, histologically proven craniopharyngiomas using immunostains for CD-34, a monoclonal antibody that selectively recognizes endothelial cells. Both histologic types of craniopharyngiomas, adamantinomatous and papillary, were included in the study. In addition, the cellular distribution of vascular endothelial growth factor (VEGF), a strong stimulator of new vessel formation, was assessed by both immunohistochemistry and in situ hybridization for VEGF receptor 2 (VEGFR-2) mRNA expression. RESULTS. Histologically, small numbers of capillaries were identified in temporal stroma but not in their epithelial components. Immunohistochemistry revealed strong, conclusive cytoplasmic immunoreactivity for VEGF in the epithelial cells of both adamantinomatous craniopharyngiomas and papillary craniopharyngiomas. In situ hybridization showed that VEGFR-2 mRNA was expressed widely, not only in neoplastic epithelium but also in capillary endothelium. CONCLUSIONS. Tumors with greater microvessel density regrow more frequently compared with tumors that have lower microvessel density, suggesting that the extent of angiogenesis is of prognostic value in patients with craniopharyngioma. VEGFR-2 may act as a key modulator of VEGF activity in endothelial cells and nonendothelial cells, indicating that VEGF plays an important role in the behavior of craniopharyngiomas.

Original languageEnglish (US)
Pages (from-to)738-745
Number of pages8
JournalCancer
Volume94
Issue number3
DOIs
StatePublished - Feb 1 2002

Fingerprint

Craniopharyngioma
Vascular Endothelial Growth Factor A
Microvessels
Vascular Endothelial Growth Factor Receptor
In Situ Hybridization
Neoplasms
Endothelial Cells
Immunohistochemistry
Vascular Endothelial Growth Factor Receptor-2
Messenger RNA
Glandular and Epithelial Neoplasms
Vascular Endothelium
Epithelium
Epithelial Cells
Monoclonal Antibodies
Recurrence

Keywords

  • Angiogenesis
  • Craniopharyngioma
  • Immunohistochemistry
  • In situ hybridization
  • Vascular endothelial growth factor
  • Vascular endothelial growth factor receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Vidal, S., Kovacs, K., Lloyd, R. V., Meyer, F. B., & Scheithauer, B. W. (2002). Angiogenesis in patients with craniopharyngiomas: Correlation with treatment and outcome. Cancer, 94(3), 738-745. https://doi.org/10.1002/cncr.10281

Angiogenesis in patients with craniopharyngiomas : Correlation with treatment and outcome. / Vidal, Sergio; Kovacs, Kalman; Lloyd, Ricardo V.; Meyer, Frederic B.; Scheithauer, Bernd W.

In: Cancer, Vol. 94, No. 3, 01.02.2002, p. 738-745.

Research output: Contribution to journalArticle

Vidal, S, Kovacs, K, Lloyd, RV, Meyer, FB & Scheithauer, BW 2002, 'Angiogenesis in patients with craniopharyngiomas: Correlation with treatment and outcome', Cancer, vol. 94, no. 3, pp. 738-745. https://doi.org/10.1002/cncr.10281
Vidal, Sergio ; Kovacs, Kalman ; Lloyd, Ricardo V. ; Meyer, Frederic B. ; Scheithauer, Bernd W. / Angiogenesis in patients with craniopharyngiomas : Correlation with treatment and outcome. In: Cancer. 2002 ; Vol. 94, No. 3. pp. 738-745.
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abstract = "BACKGROUND. Craniopharyngiomas are histologically benign epithelial neoplasms of the sellar region that often exhibit aggressive and invasive growth. The authors hypothesized that tumor proliferation, spread, and recurrence are angiogenesis dependent and investigated the significance of vascularization relative to biologic behavior. To the authors' knowledge, angiogenesis for patients with craniopharyngiomas has not been examined to date. METHODS. The authors measured microvessel densities in resected, histologically proven craniopharyngiomas using immunostains for CD-34, a monoclonal antibody that selectively recognizes endothelial cells. Both histologic types of craniopharyngiomas, adamantinomatous and papillary, were included in the study. In addition, the cellular distribution of vascular endothelial growth factor (VEGF), a strong stimulator of new vessel formation, was assessed by both immunohistochemistry and in situ hybridization for VEGF receptor 2 (VEGFR-2) mRNA expression. RESULTS. Histologically, small numbers of capillaries were identified in temporal stroma but not in their epithelial components. Immunohistochemistry revealed strong, conclusive cytoplasmic immunoreactivity for VEGF in the epithelial cells of both adamantinomatous craniopharyngiomas and papillary craniopharyngiomas. In situ hybridization showed that VEGFR-2 mRNA was expressed widely, not only in neoplastic epithelium but also in capillary endothelium. CONCLUSIONS. Tumors with greater microvessel density regrow more frequently compared with tumors that have lower microvessel density, suggesting that the extent of angiogenesis is of prognostic value in patients with craniopharyngioma. VEGFR-2 may act as a key modulator of VEGF activity in endothelial cells and nonendothelial cells, indicating that VEGF plays an important role in the behavior of craniopharyngiomas.",
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AB - BACKGROUND. Craniopharyngiomas are histologically benign epithelial neoplasms of the sellar region that often exhibit aggressive and invasive growth. The authors hypothesized that tumor proliferation, spread, and recurrence are angiogenesis dependent and investigated the significance of vascularization relative to biologic behavior. To the authors' knowledge, angiogenesis for patients with craniopharyngiomas has not been examined to date. METHODS. The authors measured microvessel densities in resected, histologically proven craniopharyngiomas using immunostains for CD-34, a monoclonal antibody that selectively recognizes endothelial cells. Both histologic types of craniopharyngiomas, adamantinomatous and papillary, were included in the study. In addition, the cellular distribution of vascular endothelial growth factor (VEGF), a strong stimulator of new vessel formation, was assessed by both immunohistochemistry and in situ hybridization for VEGF receptor 2 (VEGFR-2) mRNA expression. RESULTS. Histologically, small numbers of capillaries were identified in temporal stroma but not in their epithelial components. Immunohistochemistry revealed strong, conclusive cytoplasmic immunoreactivity for VEGF in the epithelial cells of both adamantinomatous craniopharyngiomas and papillary craniopharyngiomas. In situ hybridization showed that VEGFR-2 mRNA was expressed widely, not only in neoplastic epithelium but also in capillary endothelium. CONCLUSIONS. Tumors with greater microvessel density regrow more frequently compared with tumors that have lower microvessel density, suggesting that the extent of angiogenesis is of prognostic value in patients with craniopharyngioma. VEGFR-2 may act as a key modulator of VEGF activity in endothelial cells and nonendothelial cells, indicating that VEGF plays an important role in the behavior of craniopharyngiomas.

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