Anesthesia inhibits interferon-induced natural killer cell cytotoxicity via induction of CD8+ suppressor cells

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Anesthesia (Avertin, halothane, isoflurane, ether, or ketamine/xylazan) of mice inhibits subsequent stimulation of splenic natural killer cell (NK) cytotoxicity by interferon (IFN) treatment either in vitro and in vivo. The current data demonstrate (a) in vitro depletion of CD8+ cells from mononuclear splenocytes of anesthetized mice restored the ability of NK cells to respond in vitro to IFN stimulation and (b) coincubation of CD8+ splenocytes from anesthetized mice with CD8- splenocytes of naive mice resulted in a significant reduction of the IFN-induced stimulation of NK activity in the coculture. These results suggest that anesthesia induces CD8+ cells that suppress stimulation of NK cytotoxicity by IFN.

Original languageEnglish (US)
Pages (from-to)474-480
Number of pages7
JournalCellular Immunology
Volume151
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Natural Killer Cells
Interferons
Anesthesia
Isoflurane
Ketamine
Halothane
Coculture Techniques
Ether
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Anesthesia inhibits interferon-induced natural killer cell cytotoxicity via induction of CD8+ suppressor cells. / Markovic, Svetomir Nenad; Murasko, D. M.

In: Cellular Immunology, Vol. 151, No. 2, 1993, p. 474-480.

Research output: Contribution to journalArticle

@article{f00afbe083ad4433afde7a12017a9537,
title = "Anesthesia inhibits interferon-induced natural killer cell cytotoxicity via induction of CD8+ suppressor cells",
abstract = "Anesthesia (Avertin, halothane, isoflurane, ether, or ketamine/xylazan) of mice inhibits subsequent stimulation of splenic natural killer cell (NK) cytotoxicity by interferon (IFN) treatment either in vitro and in vivo. The current data demonstrate (a) in vitro depletion of CD8+ cells from mononuclear splenocytes of anesthetized mice restored the ability of NK cells to respond in vitro to IFN stimulation and (b) coincubation of CD8+ splenocytes from anesthetized mice with CD8- splenocytes of naive mice resulted in a significant reduction of the IFN-induced stimulation of NK activity in the coculture. These results suggest that anesthesia induces CD8+ cells that suppress stimulation of NK cytotoxicity by IFN.",
author = "Markovic, {Svetomir Nenad} and Murasko, {D. M.}",
year = "1993",
doi = "10.1006/cimm.1993.1256",
language = "English (US)",
volume = "151",
pages = "474--480",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Anesthesia inhibits interferon-induced natural killer cell cytotoxicity via induction of CD8+ suppressor cells

AU - Markovic, Svetomir Nenad

AU - Murasko, D. M.

PY - 1993

Y1 - 1993

N2 - Anesthesia (Avertin, halothane, isoflurane, ether, or ketamine/xylazan) of mice inhibits subsequent stimulation of splenic natural killer cell (NK) cytotoxicity by interferon (IFN) treatment either in vitro and in vivo. The current data demonstrate (a) in vitro depletion of CD8+ cells from mononuclear splenocytes of anesthetized mice restored the ability of NK cells to respond in vitro to IFN stimulation and (b) coincubation of CD8+ splenocytes from anesthetized mice with CD8- splenocytes of naive mice resulted in a significant reduction of the IFN-induced stimulation of NK activity in the coculture. These results suggest that anesthesia induces CD8+ cells that suppress stimulation of NK cytotoxicity by IFN.

AB - Anesthesia (Avertin, halothane, isoflurane, ether, or ketamine/xylazan) of mice inhibits subsequent stimulation of splenic natural killer cell (NK) cytotoxicity by interferon (IFN) treatment either in vitro and in vivo. The current data demonstrate (a) in vitro depletion of CD8+ cells from mononuclear splenocytes of anesthetized mice restored the ability of NK cells to respond in vitro to IFN stimulation and (b) coincubation of CD8+ splenocytes from anesthetized mice with CD8- splenocytes of naive mice resulted in a significant reduction of the IFN-induced stimulation of NK activity in the coculture. These results suggest that anesthesia induces CD8+ cells that suppress stimulation of NK cytotoxicity by IFN.

UR - http://www.scopus.com/inward/record.url?scp=0027423301&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027423301&partnerID=8YFLogxK

U2 - 10.1006/cimm.1993.1256

DO - 10.1006/cimm.1993.1256

M3 - Article

VL - 151

SP - 474

EP - 480

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 2

ER -