Anemic zebrafish models of cardiomyopathy

Xiaojing Sun, Xiaolei Xu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cardiomyopathy refers to the cardiac remodeling process in response to a variety of intrinsic and extrinsic stimuli that stress the heart. To discover novel therapeutic strategies for the disease, we are establishing and characterizing adult zebrafish models of cardiomyopathy. One of the models is tr265/tr265, a line that becomes anemic due to a mutation that ablates erythroid-specific Band 3 protein. Although Band 3 does not express in the heart, the chronic anemic stress induces profound cardiac enlargement and cardiomyopathy-like pathogenesis. Phenylhydrazine hydrochloride (PHZ)-induced anemia model has been established which enables application of anemia stress to any adult fish. In this chapter, we provide detailed information on generation of two anemic models in zebrafish; measurement of the anemia level; determination of enlarged hearts in either organ level or cellular level; and detection of cardiomyocyte hyperplasia, as well as survival rate recording. The protocols described here can be applied to other adult zebrafish models of cardiomyopathy.

Original languageEnglish (US)
Title of host publicationTRP Channels in Drug Discovery
Subtitle of host publicationVolume II
Editors Arpad Szallasi, Tamas Biro
Pages41-54
Number of pages14
DOIs
StatePublished - Nov 19 2012

Publication series

NameMethods in Pharmacology and Toxicology
ISSN (Print)1557-2153
ISSN (Electronic)1940-6053

Keywords

  • Anemia
  • Cardiomyopathy
  • Phenylhydrazine hydrochloride (PHZ)
  • Tr265
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

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