Anemic zebrafish models of cardiomyopathy

Xiaojing Sun, Xiaolei H Xu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cardiomyopathy refers to the cardiac remodeling process in response to a variety of intrinsic and extrinsic stimuli that stress the heart. To discover novel therapeutic strategies for the disease, we are establishing and characterizing adult zebrafish models of cardiomyopathy. One of the models is tr265/tr265, a line that becomes anemic due to a mutation that ablates erythroid-specific Band 3 protein. Although Band 3 does not express in the heart, the chronic anemic stress induces profound cardiac enlargement and cardiomyopathy-like pathogenesis. Phenylhydrazine hydrochloride (PHZ)-induced anemia model has been established which enables application of anemia stress to any adult fish. In this chapter, we provide detailed information on generation of two anemic models in zebrafish; measurement of the anemia level; determination of enlarged hearts in either organ level or cellular level; and detection of cardiomyocyte hyperplasia, as well as survival rate recording. The protocols described here can be applied to other adult zebrafish models of cardiomyopathy.

Original languageEnglish (US)
Title of host publicationMethods in Pharmacology and Toxicology
Pages41-54
Number of pages14
DOIs
StatePublished - 2012

Publication series

NameMethods in Pharmacology and Toxicology
ISSN (Print)15572153
ISSN (Electronic)19406053

Fingerprint

Zebrafish
Cardiomyopathies
Anemia
Erythrocyte Anion Exchange Protein 1
Cardiomegaly
Cardiac Myocytes
Hyperplasia
Fishes
Mutation
Therapeutics

Keywords

  • Anemia
  • Cardiomyopathy
  • Phenylhydrazine hydrochloride (PHZ)
  • Tr265
  • Zebrafish

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Molecular Medicine
  • Pharmacology (medical)

Cite this

Sun, X., & Xu, X. H. (2012). Anemic zebrafish models of cardiomyopathy. In Methods in Pharmacology and Toxicology (pp. 41-54). (Methods in Pharmacology and Toxicology). https://doi.org/10.1007/978-1-62703-095-3_2

Anemic zebrafish models of cardiomyopathy. / Sun, Xiaojing; Xu, Xiaolei H.

Methods in Pharmacology and Toxicology. 2012. p. 41-54 (Methods in Pharmacology and Toxicology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sun, X & Xu, XH 2012, Anemic zebrafish models of cardiomyopathy. in Methods in Pharmacology and Toxicology. Methods in Pharmacology and Toxicology, pp. 41-54. https://doi.org/10.1007/978-1-62703-095-3_2
Sun X, Xu XH. Anemic zebrafish models of cardiomyopathy. In Methods in Pharmacology and Toxicology. 2012. p. 41-54. (Methods in Pharmacology and Toxicology). https://doi.org/10.1007/978-1-62703-095-3_2
Sun, Xiaojing ; Xu, Xiaolei H. / Anemic zebrafish models of cardiomyopathy. Methods in Pharmacology and Toxicology. 2012. pp. 41-54 (Methods in Pharmacology and Toxicology).
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