Androgen receptors in rat testis

V. Hansson, W. S. McLean, A. A. Smith, D. J. Tindall, S. C. Weddington, S. N. Nayfeh, F. S. French, E. M. Ritzen

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Testosterone (T) and 5α-dihydrotestosterone (17β-hydroxy-5α-androstan-3-one; DHT) are bound to specific cytoplasmic receptors (CR) in 105, 000 × g supernatant fractions of seminiferous tubules from hypophysectomized rats following the intravenous injection of [1, 2-3h]testosterone. CR is clearly different from the testicular androgen binding protein (ABP) by electrophoretic mobility, temperature stability and rate of dissociation of steroid-CR complex, but very similar to the cytoplasmic receptors of epididymis and ventral prostate. Under these labeling conditions, the nuclei of seminiferous tubules also contain radioactive T and DHT bound to protein. These androgen-protein complexes, which can be extracted with 0.4 M - 1 M KC1, have a sedimentation coefficient of 3-4 S. Binding of radioactive T and DHT to both cytoplasmic and nuclear receptors in vivo is specific for androgen target tissues and abolished by simultaneous injection of unlabeled T, DHT and cyproterone acetate (1, 2-α-methylene-6-chloro-pregn-4, 6-diene-17α-o1-3, 20-diene-17-acetate), but not by cortisol. It is suggested that receptors for testosterone and DHT in the seminiferous tubules are involved in the mediation of the androgenic stimulus to the germ cells.

Original languageEnglish (US)
Pages (from-to)823-832
Number of pages10
JournalSteroids
Volume23
Issue number5
DOIs
StatePublished - May 1974

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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    Hansson, V., McLean, W. S., Smith, A. A., Tindall, D. J., Weddington, S. C., Nayfeh, S. N., French, F. S., & Ritzen, E. M. (1974). Androgen receptors in rat testis. Steroids, 23(5), 823-832. https://doi.org/10.1016/0039-128X(74)90169-X