Androgen receptors in rat testis

V. Hansson, W. S. McLean, A. A. Smith, D. J. Tindall, S. C. Weddington, S. N. Nayfeh, F. S. French, E. M. Ritzen

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Testosterone (T) and 5α dihydrotestosterone (17β hydroxy 5α androstan 3 one; DHT) are bound to specific cytoplasmic receptors (CR) in 105,000 x g supernatant fractions of seminiferous tubules from hypophysectomized rats following the intravenous injection of [1,2 3H]testosterone. CR is clearly different from the testicular androgen binding protein (ABP) by electrophoretic mobility, temperature stability and rate of dissociation of steroid and CR complex, but very similar to the cytoplasmic receptors of epididymis and ventral prostate. Under these labeling conditions. the nuclei of seminiferous tubules also contain radioactive T and DHT bound to protein. These androgen protein complexes, which can be extracted with 0.4 M to 1 M KCl, have a sedimentation coefficient of 3 to 4 S. Binding of radioactive T and DHT to both cytoplasmic and nuclear receptors in vivo is specific for androgen target tissues and abolished by simultaneous injection of unlabeled T, DHT and cyproterone acetate (1,2 α methylene 6 chloro pregna 4,6 diene 17α ol 3,20 diene 17 acetate), but not by cortisol. It is suggested that receptors for testosterone and DHT in the seminiferous tubules are involved in the mediation of the androgenic stimulus to the germ cells.

Original languageEnglish (US)
Pages (from-to)823-832
Number of pages10
JournalSteroids
Volume23
Issue number6
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

Androgen Receptors
Cytoplasmic and Nuclear Receptors
Testis
Rats
Seminiferous Tubules
Androgens
Testosterone
Androgen-Binding Protein
Cyproterone Acetate
Electrophoretic mobility
Dihydrotestosterone
Steroid Receptors
Sedimentation
Labeling
Epididymis
Hydrocortisone
Proteins
Acetates
Germ Cells
Intravenous Injections

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Hansson, V., McLean, W. S., Smith, A. A., Tindall, D. J., Weddington, S. C., Nayfeh, S. N., ... Ritzen, E. M. (1974). Androgen receptors in rat testis. Steroids, 23(6), 823-832. https://doi.org/10.1016/0039-128X(74)90169-X

Androgen receptors in rat testis. / Hansson, V.; McLean, W. S.; Smith, A. A.; Tindall, D. J.; Weddington, S. C.; Nayfeh, S. N.; French, F. S.; Ritzen, E. M.

In: Steroids, Vol. 23, No. 6, 1974, p. 823-832.

Research output: Contribution to journalArticle

Hansson, V, McLean, WS, Smith, AA, Tindall, DJ, Weddington, SC, Nayfeh, SN, French, FS & Ritzen, EM 1974, 'Androgen receptors in rat testis', Steroids, vol. 23, no. 6, pp. 823-832. https://doi.org/10.1016/0039-128X(74)90169-X
Hansson V, McLean WS, Smith AA, Tindall DJ, Weddington SC, Nayfeh SN et al. Androgen receptors in rat testis. Steroids. 1974;23(6):823-832. https://doi.org/10.1016/0039-128X(74)90169-X
Hansson, V. ; McLean, W. S. ; Smith, A. A. ; Tindall, D. J. ; Weddington, S. C. ; Nayfeh, S. N. ; French, F. S. ; Ritzen, E. M. / Androgen receptors in rat testis. In: Steroids. 1974 ; Vol. 23, No. 6. pp. 823-832.
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