Ancestry-shift refinement mapping of the C6orf97-ESR1 breast cancer susceptibility locus

Simon N. Stacey, Patrick Sulem, Carlo Zanon, Sigurjon A. Gudjonsson, Gudmar Thorleifsson, Agnar Helgason, Aslaug Jonasdottir, Soren Besenbacher, Jelena P. Kostic, James D. Fackenthal, Dezheng Huo, Clement Adebamowo, Temidayo Ogundiran, Janet E. Olson, Zachary S. Fredericksen, Xianshu Wang, Maxime P. Look, Anieta M. Sieuwerts, John W.M. Martens, Isabel PajaresMaria D. Garcia-Prats, Jose M. Ramon-Cajal, Ana de Juan, Angeles Panadero, Eugenia Ortega, Katja K.H. Aben, Sita H. Vermeulen, Fatemeh Asadzadeh, K. C. Anton van Engelenburg, Sara Margolin, Chen Yang Shen, Pei Ei Wu, Asta Försti, Per Lenner, Roger Henriksson, Robert Johansson, Kerstin Enquist, Göran Hallmans, Thorvaldur Jonsson, Helgi Sigurdsson, Kristin Alexiusdottir, Julius Gudmundsson, Asgeir Sigurdsson, Michael L. Frigge, Larus Gudmundsson, Kristleifur Kristjansson, Bjarni V. Halldorsson, Unnur Styrkarsdottir, Jeffrey R. Gulcher, Kari Hemminki, Annika Lindblom, Lambertus A. Kiemeney, Jose I. Mayordomo, John A. Foekens, Fergus J. Couch, Olufunmilayo I. Olopade, Daniel F. Gudbjartsson, Unnur Thorsteinsdottir, Thorunn Rafnar, Oskar T. Johannsson, Kari Stefansson

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Abstract

We used an approach that we term ancestry-shift refinement mapping to investigate an association, originally discovered in a GWAS of a Chinese population, between rs2046210[T] and breast cancer susceptibility. The locus is on 6q25.1 in proximity to the C6orf97 and estrogen receptor α (ESR1) genes. We identified a panel of SNPs that are correlated with rs2046210 in Chinese, but not necessarily so in other ancestral populations, and genotyped them in breast cancer case:control samples of Asian, European, and African origin, a total of 10,176 cases and 13,286 controls. We found that rs2046210[T] does not confer substantial risk of breast cancer in Europeans and Africans (OR = 1.04, P = 0.099, and OR = 0.98, P = 0.77, respectively). Rather, in those ancestries, an association signal arises from a group of less common SNPs typified by rs9397435. The rs9397435[G] allele was found to confer risk of breast cancer in European (OR = 1.15, P = 1.2×10-3), African (OR = 1.35, P = 0.014), and Asian (OR = 1.23, P = 2.9×10-4) population samples. Combined over all ancestries, the OR was 1.19 (P = 3.9×10-7), was without significant heterogeneity between ancestries (Phet = 0.36) and the SNP fully accounted for the association signal in each ancestry. Haplotypes bearing rs9397435[G] are well tagged by rs2046210[T] only in Asians. The rs9397435[G] allele showed associations with both estrogen receptor positive and estrogen receptor negative breast cancer. Using early-draft data from the 1,000 Genomes project, we found that the risk allele of a novel SNP (rs77275268), which is closely correlated with rs9397435, disrupts a partially methylated CpG sequence within a known CTCF binding site. These studies demonstrate that shifting the analysis among ancestral populations can provide valuable resolution in association mapping.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalPLoS genetics
Volume6
Issue number7
DOIs
StatePublished - Jul 2010

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

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    Stacey, S. N., Sulem, P., Zanon, C., Gudjonsson, S. A., Thorleifsson, G., Helgason, A., Jonasdottir, A., Besenbacher, S., Kostic, J. P., Fackenthal, J. D., Huo, D., Adebamowo, C., Ogundiran, T., Olson, J. E., Fredericksen, Z. S., Wang, X., Look, M. P., Sieuwerts, A. M., Martens, J. W. M., ... Stefansson, K. (2010). Ancestry-shift refinement mapping of the C6orf97-ESR1 breast cancer susceptibility locus. PLoS genetics, 6(7), 1-12. https://doi.org/10.1371/journal.pgen.1001029