Analysis of transforming growth factor β and other cytokines in autoimmune exocrinopathy (Sjogren's syndrome)

N. Ogawa, H. Dang, Konstantinos N Lazaridis, H. S. McGuff, T. B. Aufdemorte, N. Talal

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Cytokines play a major role in tissue destruction caused by autoimmune dysregulation. In Sjogren's syndrome (SS) patients, salivary glands are the target organs for autoimmune tissue damage. In the present study, reverse trancriptase-polymerase chain reaction (RT-PCR) was used to look for cytokine mRNA expressed in SS salivary glands. Focus score was used to determine the severity of the lesions. Cytokine production in supernatants of the salivary gland cell culture was measured by enzyme-linked immunosorbent assay (ELISA). Immunohistochemical staining was used to identify the local presence of transforming growth factor β (TGF-β). Interleukin (IL)-2, IL-6, and IL-10 mRNA were expressed in moderate to severe SS salivary gland lesions. TGF-β mRNA was constitutively expressed in normal and SS salivary glands. In SS salivary gland cell cultures, IL-6 and IL-10 proteins were produced. TGF-β production was reduced in high focus score SS glands. Normal and minimally involved SS salivary gland ductal epithelium and acinar cells were found to produce TGF-β by immunostaining. In conclusion, an excess production of IL- 2, IL-6, and IL-10 and a reduced production of the immunosuppressive cytokine, TGF-β, may he responsible for the progression of the salivary gland lesion in SS. Specific immunotherapy can now be designed based on mechanisms to correct this cytokine imbalance and benefit patients with autoimmune diseases, such as SS.

Original languageEnglish (US)
Pages (from-to)759-767
Number of pages9
JournalJournal of Interferon and Cytokine Research
Volume15
Issue number9
StatePublished - 1995
Externally publishedYes

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Sjogren's Syndrome
Transforming Growth Factors
Salivary Glands
Cytokines
Interleukin-10
Interleukin-6
Messenger RNA
Interleukin-2
Cell Culture Techniques
Acinar Cells
Immunosuppressive Agents
Immunotherapy
Autoimmune Diseases
Epithelium
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Virology

Cite this

Analysis of transforming growth factor β and other cytokines in autoimmune exocrinopathy (Sjogren's syndrome). / Ogawa, N.; Dang, H.; Lazaridis, Konstantinos N; McGuff, H. S.; Aufdemorte, T. B.; Talal, N.

In: Journal of Interferon and Cytokine Research, Vol. 15, No. 9, 1995, p. 759-767.

Research output: Contribution to journalArticle

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