Analysis of the phosphofructokinase subunits and isoenzymes in human tissues

G. A. Dunaway, T. P. Kasten, T. Sebo, R. Trapp

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

The 6-phosphofructo-1-kinase (PFK) subunits and isoenzymes were studied in human muscle, heart, brain, liver, platelets, fibroblasts, erythrocytes, placenta and umbilical cord. In each tissue, the subunit types in the native isoenzymes were characterized by immunological titration with subunit-specific antibodies and by column chromatography on QAE (quaternary aminoethyl)-Sephadex. Further, the subunits of the partially purified native isoenzymes were resolved by SDS/polyacrylamide-gel electrophoresis, identified by immunoblotting, and quantified by scanning gel densitometry of silver-stained gels and immunoblots. Depending on the type of tissue, one to three subunits were detected. The M(r) values of the L, M and C subunits regardless of tissue were 76,700 ± 1400, 82,500 ± 1640 and 86,500 ± 1620. Of the tissues studied, only the muscle PFK isoenzymes exhibited one subunit, which was the M-type subunit. Of the other tissues studied, the PFK isoenzymes contained various amounts of all three subunits. Considering the properties of the native PFK isoenzymes, it is clear that, in human tissues, they are not simply various combinations of two or three homotetramic isoenzymes, but complex mixtures of homotetramers and heterotetramers. The kinetic/regulatory properties of the various isoenzyme pools were found to be dependent on subunit composition.

Original languageEnglish (US)
Pages (from-to)677-683
Number of pages7
JournalBiochemical Journal
Volume251
Issue number3
StatePublished - 1988
Externally publishedYes

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Phosphofructokinases
Isoenzymes
Phosphofructokinase-1
Tissue
Muscle
Gels
Column chromatography
Densitometry
Umbilical Cord
Fibroblasts
Platelets
Electrophoresis
Complex Mixtures
Titration
Silver
Immunoblotting
Liver
Placenta
Chromatography
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dunaway, G. A., Kasten, T. P., Sebo, T., & Trapp, R. (1988). Analysis of the phosphofructokinase subunits and isoenzymes in human tissues. Biochemical Journal, 251(3), 677-683.

Analysis of the phosphofructokinase subunits and isoenzymes in human tissues. / Dunaway, G. A.; Kasten, T. P.; Sebo, T.; Trapp, R.

In: Biochemical Journal, Vol. 251, No. 3, 1988, p. 677-683.

Research output: Contribution to journalArticle

Dunaway, GA, Kasten, TP, Sebo, T & Trapp, R 1988, 'Analysis of the phosphofructokinase subunits and isoenzymes in human tissues', Biochemical Journal, vol. 251, no. 3, pp. 677-683.
Dunaway GA, Kasten TP, Sebo T, Trapp R. Analysis of the phosphofructokinase subunits and isoenzymes in human tissues. Biochemical Journal. 1988;251(3):677-683.
Dunaway, G. A. ; Kasten, T. P. ; Sebo, T. ; Trapp, R. / Analysis of the phosphofructokinase subunits and isoenzymes in human tissues. In: Biochemical Journal. 1988 ; Vol. 251, No. 3. pp. 677-683.
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