TY - JOUR
T1 - Analysis of the phosphofructokinase subunits and isoenzymes in human tissues
AU - Dunaway, G. A.
AU - Kasten, T. P.
AU - Sebo, T.
AU - Trapp, R.
PY - 1988
Y1 - 1988
N2 - The 6-phosphofructo-1-kinase (PFK) subunits and isoenzymes were studied in human muscle, heart, brain, liver, platelets, fibroblasts, erythrocytes, placenta and umbilical cord. In each tissue, the subunit types in the native isoenzymes were characterized by immunological titration with subunit-specific antibodies and by column chromatography on QAE (quaternary aminoethyl)-Sephadex. Further, the subunits of the partially purified native isoenzymes were resolved by SDS/polyacrylamide-gel electrophoresis, identified by immunoblotting, and quantified by scanning gel densitometry of silver-stained gels and immunoblots. Depending on the type of tissue, one to three subunits were detected. The M(r) values of the L, M and C subunits regardless of tissue were 76,700 ± 1400, 82,500 ± 1640 and 86,500 ± 1620. Of the tissues studied, only the muscle PFK isoenzymes exhibited one subunit, which was the M-type subunit. Of the other tissues studied, the PFK isoenzymes contained various amounts of all three subunits. Considering the properties of the native PFK isoenzymes, it is clear that, in human tissues, they are not simply various combinations of two or three homotetramic isoenzymes, but complex mixtures of homotetramers and heterotetramers. The kinetic/regulatory properties of the various isoenzyme pools were found to be dependent on subunit composition.
AB - The 6-phosphofructo-1-kinase (PFK) subunits and isoenzymes were studied in human muscle, heart, brain, liver, platelets, fibroblasts, erythrocytes, placenta and umbilical cord. In each tissue, the subunit types in the native isoenzymes were characterized by immunological titration with subunit-specific antibodies and by column chromatography on QAE (quaternary aminoethyl)-Sephadex. Further, the subunits of the partially purified native isoenzymes were resolved by SDS/polyacrylamide-gel electrophoresis, identified by immunoblotting, and quantified by scanning gel densitometry of silver-stained gels and immunoblots. Depending on the type of tissue, one to three subunits were detected. The M(r) values of the L, M and C subunits regardless of tissue were 76,700 ± 1400, 82,500 ± 1640 and 86,500 ± 1620. Of the tissues studied, only the muscle PFK isoenzymes exhibited one subunit, which was the M-type subunit. Of the other tissues studied, the PFK isoenzymes contained various amounts of all three subunits. Considering the properties of the native PFK isoenzymes, it is clear that, in human tissues, they are not simply various combinations of two or three homotetramic isoenzymes, but complex mixtures of homotetramers and heterotetramers. The kinetic/regulatory properties of the various isoenzyme pools were found to be dependent on subunit composition.
UR - http://www.scopus.com/inward/record.url?scp=0023918653&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023918653&partnerID=8YFLogxK
U2 - 10.1042/bj2510677
DO - 10.1042/bj2510677
M3 - Article
C2 - 2970843
AN - SCOPUS:0023918653
SN - 0264-6021
VL - 251
SP - 677
EP - 683
JO - Biochemical Journal
JF - Biochemical Journal
IS - 3
ER -