Analysis of PTEN/MMAC1 alterations in aerodigestive tract tumors

Kenji Okami, Li Wu, Greg Riggins, Paul Cairns, Michael Goggins, Ella Evron, Naomi Halachmi, Steven A. Ahrendt, Andre L. Reed, Werner Hilgers, Scott E. Kern, Wayne M. Koch, David Sidransky, Jin Jen

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

PTEN/MMAC1 is a candidate tumor suppressor gene recently identified at chromosomal band 10q23. It is mutated in sporadic brain, breast, and prostate cancer and in the germ line of patients with hereditary Cowden disease. We searched for genetic alterations of the PTEN/MMAC1 gene in 39 primary head and neck cancers (HNSCCs), 42 primary non-small cell lung cancers (NSCLCs), 80 pancreatic cancer xenografts, and 37 cell lines and xenografts from colon, lung, and gastric cancers. Microsatellite analysis revealed loss of heterozygosity at markers near the gene in 41% of primary HNSCCs, 50% of NSCLCs, and 39% of the pancreatic cancers. Three cases of HNSCCs displayed homozygous deletion involving the gene. We sequenced the entire coding region of the PTEN/MMAC1 gene in the remaining tumors displaying loss of heterozygosity and found one terminating mutation in a HNSCC sample. Thus, a second inactivation event was observed in 4 of 39 primary HNSCC cases. By use of a protein truncation assay, one terminating mutation was also identified in one of eight NSCLC cell lines. Our results suggest that PTEN/MMAC1 gene inactivation plays o role in the genesis of some tumor types.

Original languageEnglish (US)
Pages (from-to)509-511
Number of pages3
JournalCancer research
Volume58
Issue number3
StatePublished - Feb 1 1998

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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