Analysis of proteins and proteomes by mass spectrometry

M. Mann, R. C. Hendrickson, Akhilesh Pandey

Research output: Contribution to journalReview article

866 Citations (Scopus)

Abstract

A decade after the discovery of electrospray and matrix-assisted laser desorption ionization (MALDI), methods that finally allowed gentle ionization of large biomolecules, mass spectrometry has become a powerful tool in protein analysis and the key technology in the emerging field of proteomics. The success of mass spectrometry is driven both by innovative instrumentation designs, especially those operating on the time-of-flight or ion-trapping principles, and by large-scale biochemical strategies, which use mass spectrometry to detect the isolated proteins. Any human protein can now be identified directly from genome databases on the basis of minimal data derived by mass spectrometry. As has already happened in genomics, increased automation of sample handling, analysis, and the interpretation of results will generate an avalanche of qualitative and quantitative proteomic data. Protein-protein interactions can be analyzed directly by precipitation of a tagged bait followed by mass spectrometric identification of its binding partners. By these and similar strategies, entire protein complexes, signaling pathways, and whole organelles are being characterized. Posttranslational modifications remain difficult to analyze but are starting to yield to generic strategies.

Original languageEnglish (US)
Pages (from-to)437-473
Number of pages37
JournalAnnual Review of Biochemistry
Volume70
DOIs
StatePublished - Jul 30 2001
Externally publishedYes

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Proteome
Mass spectrometry
Mass Spectrometry
Proteins
Proteomics
Ionization
Avalanches
Automation
Biomolecules
Post Translational Protein Processing
Genomics
Organelles
Desorption
Lasers
Genes
Genome
Databases
Ions
Technology

Keywords

  • Functional genomics
  • Phosphorylation
  • Posttranslational modifications
  • Protein interaction
  • Proteomics
  • Signal transduction

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry

Cite this

Analysis of proteins and proteomes by mass spectrometry. / Mann, M.; Hendrickson, R. C.; Pandey, Akhilesh.

In: Annual Review of Biochemistry, Vol. 70, 30.07.2001, p. 437-473.

Research output: Contribution to journalReview article

Mann, M. ; Hendrickson, R. C. ; Pandey, Akhilesh. / Analysis of proteins and proteomes by mass spectrometry. In: Annual Review of Biochemistry. 2001 ; Vol. 70. pp. 437-473.
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