Analysis of protein phosphorylation using mass spectrometry: Deciphering the phosphoproteome

Matthias Mann, Shao En Ong, Mads Grønborg, Hanno Steen, Ole N. Jensen, Akhilesh Pandey

Research output: Contribution to journalReview article

723 Citations (Scopus)

Abstract

In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand signaling processes, we must first acquire an inventory of phosphoproteins and their phosphorylation sites under different conditions. Because phosphorylation is a dynamic process, elucidation of signaling networks also requires quantitation of these phosphorylation events. In this article, we outline several methods for enrichment of phosphorylated proteins and peptides and discuss various options for their identification and quantitation with special emphasis on mass spectrometry-based techniques.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalTrends in Biotechnology
Volume20
Issue number6
DOIs
StatePublished - Jun 1 2002
Externally publishedYes

Fingerprint

Phosphorylation
Mass spectrometry
Mass Spectrometry
Proteins
Signal transduction
Phosphoproteins
Eukaryota
Peptides
Signal Transduction
Equipment and Supplies

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Analysis of protein phosphorylation using mass spectrometry : Deciphering the phosphoproteome. / Mann, Matthias; Ong, Shao En; Grønborg, Mads; Steen, Hanno; Jensen, Ole N.; Pandey, Akhilesh.

In: Trends in Biotechnology, Vol. 20, No. 6, 01.06.2002, p. 261-268.

Research output: Contribution to journalReview article

Mann, Matthias ; Ong, Shao En ; Grønborg, Mads ; Steen, Hanno ; Jensen, Ole N. ; Pandey, Akhilesh. / Analysis of protein phosphorylation using mass spectrometry : Deciphering the phosphoproteome. In: Trends in Biotechnology. 2002 ; Vol. 20, No. 6. pp. 261-268.
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