Analysis of polyomavirus-infected renal transplant recipients' urine specimens: Correlation of routine urine cytology, fluorescence in situ hybridization, and digital image analysis

Benjamin R. Kipp, Thomas J. Sebo, Matthew D. Griffin, Johnita M. Ihrke, Kevin C. Halling

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Urinary polyomavirus subtype BK (BKV)-infected urothelial and renal tubular cells, "decoy cells," have been shown to be aneuploid with digital image analysis (DIA). We wanted to determine whether decoy cells cause false-positive fluorescence in situ hybridization (FISH) results in BKV-infected urine samples. Urine samples from 38 renal transplant recipients were split 3 ways and evaluated for number of decoy cells per 10 high-power fields by routine cytology, total nuclear DNA content by DIA, and chromosomal abnormalities by FISH. For DIA, Feulgen-stained cells were quantified with a CAS 200 image analyzer (Bacus Laboratories, Lombard, IL), and for FISH, the UroVysion probe set (Vysis, Downers Grove, IL) consisting of chromosome enumeration probes 3, 7, 17, and locus-specific identifier probe 9p21 (p16 gene) was used. Of the 38 specimens, 32 (84.2%) had evidence of BKV infection by routine cytology. DIA and FISH results were aneuploid/aneusomic in 30 (93.8%) and 4 (12.5%) cases, respectively. All aneusomic FISH specimens occurred in men older than 52 years of age and who also had 4 of the 6 highest PCR-BKV titers. To date, no patient has clinical evidence of malignancy. The 6 specimens without decoy cells were DNA diploid/disomic by DIA and FISH. Abnormal FISH results of urinary decoy cells occur much less frequently than aneuploidy by DIA in renal transplant recipients and might merit close follow-up in some transplant recipients.

Original languageEnglish (US)
Pages (from-to)854-861
Number of pages8
JournalAmerican journal of clinical pathology
Volume124
Issue number6
DOIs
StatePublished - Dec 2005

Keywords

  • Decoy cell
  • FISH
  • Fluorescence in situ hybridization
  • Image cytometry
  • UroVysion

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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