Analysis of phosphor amide mustard by reversed-phase ion pair high pressure liquid chromatography

R. W. Hardy, M. J. Moore, C. Erlichman, S. J. Soldin

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Abstract

The analysis of phosphoramide mustard by reversed-phase ion pair high pressure liquid chromatography using ultraviolet detection is described. The method will enable measurement of serum concentrations of phosphoramide mustard over a period of ~24 h after an i.v. dose of 60 mg/kg of cyclophosphamide and requires 0.5 ml of serum or plasma. The between-day precision of the assay at concentrations of 0.34, 2.76, and 6.90 mg/L gave coefficients of variation of 16, 10, and 9%, respectively. The percentage analytical recovery of phosphoramide mustard is 35%. Preliminary results providing pharmacokinetic data on two adults undergoing high-dose cyclophosphamide treatment prior to bone marrow transplant are presented.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalTherapeutic Drug Monitoring
Volume9
Issue number2
DOIs
StatePublished - Jun 1987

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Keywords

  • Ion pair
  • Phosphoramide mustard
  • Serum concentrations

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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