Analysis of Panax notoginseng metabolites in rat bile by liquid chromatography-quadrupole time-of-flight mass spectrometry with microdialysis sampling

Xiao Dong Wen, Jie Yang, Rong Hua Ma, Wen Gao, Lian Wen Qi, Ping Li, Brent A. Bauer, Guang Jian Du, Zhiyu Zhang, Jacqueline Somogyi, Chong Zhi Wang, Chun Su Yuan

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

A dynamic microdialysis sampling method with liquid chromatography-quadrupole time-of-flight mass spectrometry (Q-TOF-MS) was developed for rapid and sensitive analysis of the metabolite profile of Panax notoginseng extract (PNE) in rat bile. In vivo studies in male Sprague-Dawley rats were performed with microdialysis probes implanted into the bile duct before bile samples were collected from 0 to 12. h. Metabolites of PNE were identified using dynamic adjustment of the fragmentor voltage to produce structure-relevant fragment ions. The mass accuracy of precursor and fragment ions was typically within 5. ppm of the theoretical values. We identified 7 compounds: 4 parent compounds (notoginsenoside R1, ginsenosides Rg1, Rb1, and Rd) and 3 metabolites (ginsenosides Rg2, Rh2, and compound K). Data from this study suggest that this microdialysis technique could be used in notoginseng saponin metabolic animal studies.

Original languageEnglish (US)
Pages (from-to)162-168
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume895-896
DOIs
StatePublished - May 1 2012

Keywords

  • Bile
  • Ginsenosides
  • Liquid chromatography-quadrupole time-of-flight mass spectrometry
  • Metabolites
  • Microdialysis sampling
  • Panax notoginseng

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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