Analysis of p53-RNA interactions in cultured human cells

Kasandra J.L. Riley, L. James Maher

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Tumor suppressor p53 is a well-characterized transcription factor that binds DNA. More enigmatic are the RNA-binding properties of p53 and their physiological relevance. We used three sensitive co-immunoprecipitation methods in an attempt to detect RNAs that tightly associate with p53 in cultured human cells. Although recombinant p53 protein binds RNA in a sequence-nonspecific mode, we do not detect specific in vivo RNA binding by p53. These results suggest that RNA binding is prevented by post-translational p53 modifications. A ribonucleoprotein (not p53) is purified by multiple IgG monoclonal antibodies (including anti-p53 antibodies) from both p53 +/+ and p53 null cells. Caution is therefore required in interpreting RNA co-immunoprecipitation experiments. Though not formally excluded, these results do not support models in which p53 binds specific RNA partners in vivo.

Original languageEnglish (US)
Pages (from-to)381-387
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume363
Issue number2
DOIs
StatePublished - Nov 16 2007

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Keywords

  • CLIP
  • Formaldehyde
  • RNA
  • UV-cross-linking
  • p53

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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