Analysis of p53-RNA interactions in cultured human cells

K. J L Riley, L James Maher III

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Tumor suppressor p53 is a well-characterized transcription factor that binds DNA. More enigmatic are the RNA-binding properties of p53 and their physiological relevance. We used three sensitive co-immunoprecipitation methods in an attempt to detect RNAs that tightly associate with p53 in cultured human cells. Although recombinant p53 protein binds RNA in a sequence-nonspecific mode, we do not detect specific in vivo RNA binding by p53. These results suggest that RNA binding is prevented by post-translational p53 modifications. A ribonucleoprotein (not p53) is purified by multiple IgG monoclonal antibodies (including anti-p53 antibodies) from both p53 +/+ and p53 null cells. Caution is therefore required in interpreting RNA co-immunoprecipitation experiments. Though not formally excluded, these results do not support models in which p53 binds specific RNA partners in vivo.

Original languageEnglish (US)
Pages (from-to)381-387
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume363
Issue number2
DOIs
StatePublished - Nov 16 2007

Fingerprint

Cultured Cells
Cells
RNA
Immunoprecipitation
Recombinant proteins
Null Lymphocytes
Ribonucleoproteins
Post Translational Protein Processing
Recombinant Proteins
Tumors
Anti-Idiotypic Antibodies
Transcription Factors
Immunoglobulin G
Monoclonal Antibodies
Antibodies
DNA
Neoplasms
Experiments

Keywords

  • CLIP
  • Formaldehyde
  • p53
  • RNA
  • UV-cross-linking

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Analysis of p53-RNA interactions in cultured human cells. / Riley, K. J L; Maher III, L James.

In: Biochemical and Biophysical Research Communications, Vol. 363, No. 2, 16.11.2007, p. 381-387.

Research output: Contribution to journalArticle

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