Analysis of nonequilibrium dynamics of bound, free, and total plasma ligand concentrations over time following nonlinear secretory inputs: Kinetics of two or more hormones pulsed into compartments containing multiple variable-affinity binding proteins

Johannes D. Veldhuis; Lindsay M. Faunt; Michael L. Johnson

doi:10.1016/S0076-6879(94)40055-5

Analysis of nonequilibrium dynamics of bound, free, and total plasma ligand concentrations over time following nonlinear secretory inputs: Kinetics of two or more hormones pulsed into compartments containing multiple variable-affinity binding proteins

Johannes D. Veldhuis, Lindsay M. Faunt, Michael L. Johnson

Endocrinology, Diabetes, Metabolism, and Nutrition

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Equations (1) through (8) provide a general mathematical model of combined pulsatile and basal effector molecule secretion, distribution, association, dissociation, and metabolic removal. Independent and interactive dynamics of multiple effector molecules and multiple distinct acceptor/transport proteins can be modeled explicitly to test relevant hypotheses of ligand binding and clearance.

Original language	English (US)
Pages (from-to)	349-377
Number of pages	29
Journal	Methods in enzymology
Volume	240
Issue number	C
DOIs	https://doi.org/10.1016/S0076-6879(94)40055-5
State	Published - Jan 1 1994

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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Veldhuis, J. D., Faunt, L. M., & Johnson, M. L. (1994). Analysis of nonequilibrium dynamics of bound, free, and total plasma ligand concentrations over time following nonlinear secretory inputs: Kinetics of two or more hormones pulsed into compartments containing multiple variable-affinity binding proteins. Methods in enzymology, 240(C), 349-377. https://doi.org/10.1016/S0076-6879(94)40055-5

Analysis of nonequilibrium dynamics of bound, free, and total plasma ligand concentrations over time following nonlinear secretory inputs : Kinetics of two or more hormones pulsed into compartments containing multiple variable-affinity binding proteins. / Veldhuis, Johannes D.; Faunt, Lindsay M.; Johnson, Michael L.

In: Methods in enzymology, Vol. 240, No. C, 01.01.1994, p. 349-377.

Research output: Contribution to journal › Article › peer-review

Veldhuis, JD, Faunt, LM & Johnson, ML 1994, 'Analysis of nonequilibrium dynamics of bound, free, and total plasma ligand concentrations over time following nonlinear secretory inputs: Kinetics of two or more hormones pulsed into compartments containing multiple variable-affinity binding proteins', Methods in enzymology, vol. 240, no. C, pp. 349-377. https://doi.org/10.1016/S0076-6879(94)40055-5

Veldhuis JD, Faunt LM, Johnson ML. Analysis of nonequilibrium dynamics of bound, free, and total plasma ligand concentrations over time following nonlinear secretory inputs: Kinetics of two or more hormones pulsed into compartments containing multiple variable-affinity binding proteins. Methods in enzymology. 1994 Jan 1;240(C):349-377. doi: 10.1016/S0076-6879(94)40055-5

Veldhuis, Johannes D. ; Faunt, Lindsay M. ; Johnson, Michael L. / Analysis of nonequilibrium dynamics of bound, free, and total plasma ligand concentrations over time following nonlinear secretory inputs : Kinetics of two or more hormones pulsed into compartments containing multiple variable-affinity binding proteins. In: Methods in enzymology. 1994 ; Vol. 240, No. C. pp. 349-377.

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abstract = "Equations (1) through (8) provide a general mathematical model of combined pulsatile and basal effector molecule secretion, distribution, association, dissociation, and metabolic removal. Independent and interactive dynamics of multiple effector molecules and multiple distinct acceptor/transport proteins can be modeled explicitly to test relevant hypotheses of ligand binding and clearance.",

author = "Veldhuis, {Johannes D.} and Faunt, {Lindsay M.} and Johnson, {Michael L.}",

note = "Funding Information: We thank Patsy Craig for skillful preparation of the manuscript and Paula P. Azimi for the artwork. This work was supported in part by National Institutes of Health Grant RR 00847 to the Clinical Research Center of the University of Virginia, Research Career Development Award 1 KO4 HD00634 (J.D.V.), NIH Grant GM-28928 (M.L.J.), the Diabetes and Endocrinology Research Center Grant NIH DK-38942, the NIH-supported Clinfo Data Reduction Systems, the Pratt Foundation, the University of Virginia Academic Enhancement Fund, and the National Science Foundation Center for Biological Timing (NSF Grant DIR89-20162).",

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AB - Equations (1) through (8) provide a general mathematical model of combined pulsatile and basal effector molecule secretion, distribution, association, dissociation, and metabolic removal. Independent and interactive dynamics of multiple effector molecules and multiple distinct acceptor/transport proteins can be modeled explicitly to test relevant hypotheses of ligand binding and clearance.

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Analysis of nonequilibrium dynamics of bound, free, and total plasma ligand concentrations over time following nonlinear secretory inputs: Kinetics of two or more hormones pulsed into compartments containing multiple variable-affinity binding proteins

Abstract

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