Analysis of necroptosis in bone marrow-derived macrophages

Diana Legarda, Adrian T. Ting

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Necroptosis in macrophages is increasingly being recognized as a significant contributor to the inflammation seen in many pathologies. Here we describe a well-known method to obtain quiescent, mature macrophages that can be used to study the molecular mechanisms that regulate necroptosis. We also describe two ways to quantify this form of programmed cell death.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages63-70
Number of pages8
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1857
ISSN (Print)1064-3745

Keywords

  • ATP viability assay
  • Bone marrow-derived macrophages
  • Cell death
  • Luminescence
  • Necroptosis
  • Propidium iodide exclusion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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