TY - GEN
T1 - Analysis of mylenated nerve fibers using an image acquisition system
AU - Johnson, David M.
AU - Wehde, Mark B.
AU - Dyck, Peter J.
AU - Engelsted, Janean K.
AU - Davies, Jenny L.
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/19
Y1 - 2017/7/19
N2 - A system to evaluate and follow morphologic changes in number, size, shape and other histological measurements of transverse myelinated fiber profiles in nerves and fiber tracts has proven to be a useful analysis tool. Applications of the system include the enumeration and sizing of peripheral nerves and central nervous system tracts for the morphologic study of development, aging, regeneration, neurotoxicity, pathologic conditions, also, the morphometric assessment of intra-epidermal nerve fiber densities, IENFD. The primary aim of the system is to provide both an operator-interactive and automated method for the detection, sizing and measurement of myelinated fibers. The system, Imaging System for Nerve Morphometry, was shown, to provide the specific functionality required to perform morphometric studies of peripheral nerve, compared to commercial systems which perform a more general estimate of histological features. The system consists of the tissue preparation process, a video camera-ready microscope capable of various resolutions, and a computer system for image acquisition, data analysis, data display and statistical analysis. The morphometric measurements obtained from this system have been used for quality control by clinical nerve pathologists, in the study of human peripheral neuropathies and the clinical assessment of IENFDs. In assessment of test retest reproducibility of periodically-sampled nerves, high degrees of reproducibility were achieved for most morphometric measurements.
AB - A system to evaluate and follow morphologic changes in number, size, shape and other histological measurements of transverse myelinated fiber profiles in nerves and fiber tracts has proven to be a useful analysis tool. Applications of the system include the enumeration and sizing of peripheral nerves and central nervous system tracts for the morphologic study of development, aging, regeneration, neurotoxicity, pathologic conditions, also, the morphometric assessment of intra-epidermal nerve fiber densities, IENFD. The primary aim of the system is to provide both an operator-interactive and automated method for the detection, sizing and measurement of myelinated fibers. The system, Imaging System for Nerve Morphometry, was shown, to provide the specific functionality required to perform morphometric studies of peripheral nerve, compared to commercial systems which perform a more general estimate of histological features. The system consists of the tissue preparation process, a video camera-ready microscope capable of various resolutions, and a computer system for image acquisition, data analysis, data display and statistical analysis. The morphometric measurements obtained from this system have been used for quality control by clinical nerve pathologists, in the study of human peripheral neuropathies and the clinical assessment of IENFDs. In assessment of test retest reproducibility of periodically-sampled nerves, high degrees of reproducibility were achieved for most morphometric measurements.
KW - imaging system
KW - morphometric
KW - myelinated fiber
KW - peripheral neuropathy
UR - http://www.scopus.com/inward/record.url?scp=85027858438&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027858438&partnerID=8YFLogxK
U2 - 10.1109/MeMeA.2017.7985848
DO - 10.1109/MeMeA.2017.7985848
M3 - Conference contribution
AN - SCOPUS:85027858438
T3 - 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings
SP - 51
EP - 55
BT - 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 12th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017
Y2 - 7 May 2017 through 10 May 2017
ER -