Analysis of lipid droplet dynamics and functions in drosophila melanogaster

Han Lee, Ying Peng, Yi Guo

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The lipid droplet (LD) is a unique cellular organelle containing a neutral-lipid core enclosed by a phospholipid monolayer and associated proteins. Despite the important function of LDs at the hub of cellular energy homeostasis regulation, major questions in the field of LD biology are still unanswered. Drosophila melanogaster has been used as a model organism to make fundamental discoveries in biology for over a century. In recent years, genome-wide unbiased reverse genetic screens using Drosophila cells or transgenic lines have been proven to provide valuable knowledge to the field of LD biology. Here we summarize the methods we use for functional genomic screens in Drosophila S2 cells to identify genes involved in LD biology, and the methods used for studying LD function in vivo using Drosophila as a model to combat metabolic diseases.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc
Pages53-69
Number of pages17
DOIs
StatePublished - 2013

Publication series

NameMethods in Cell Biology
Volume116
ISSN (Print)0091-679X

Keywords

  • Drosophila melanogaster
  • Fat body
  • Genome-wide RNA interference screen
  • Lipid droplet

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Analysis of lipid droplet dynamics and functions in drosophila melanogaster'. Together they form a unique fingerprint.

  • Cite this

    Lee, H., Peng, Y., & Guo, Y. (2013). Analysis of lipid droplet dynamics and functions in drosophila melanogaster. In Methods in Cell Biology (pp. 53-69). (Methods in Cell Biology; Vol. 116). Academic Press Inc. https://doi.org/10.1016/B978-0-12-408051-5.00004-8