Analysis of lipid droplet dynamics and functions in drosophila melanogaster

Han Lee, Ying Peng, Yi D Guo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The lipid droplet (LD) is a unique cellular organelle containing a neutral-lipid core enclosed by a phospholipid monolayer and associated proteins. Despite the important function of LDs at the hub of cellular energy homeostasis regulation, major questions in the field of LD biology are still unanswered. Drosophila melanogaster has been used as a model organism to make fundamental discoveries in biology for over a century. In recent years, genome-wide unbiased reverse genetic screens using Drosophila cells or transgenic lines have been proven to provide valuable knowledge to the field of LD biology. Here we summarize the methods we use for functional genomic screens in Drosophila S2 cells to identify genes involved in LD biology, and the methods used for studying LD function in vivo using Drosophila as a model to combat metabolic diseases.

Original languageEnglish (US)
Pages (from-to)53-69
Number of pages17
JournalMethods in Cell Biology
Volume116
DOIs
StatePublished - 2013

Fingerprint

Drosophila melanogaster
Drosophila
Reverse Genetics
Metabolic Diseases
Organelles
Phospholipids
Homeostasis
Lipid Droplets
Genome
Lipids
Genes
Proteins

Keywords

  • Drosophila melanogaster
  • Fat body
  • Genome-wide RNA interference screen
  • Lipid droplet

ASJC Scopus subject areas

  • Cell Biology

Cite this

Analysis of lipid droplet dynamics and functions in drosophila melanogaster. / Lee, Han; Peng, Ying; Guo, Yi D.

In: Methods in Cell Biology, Vol. 116, 2013, p. 53-69.

Research output: Contribution to journalArticle

Lee, Han ; Peng, Ying ; Guo, Yi D. / Analysis of lipid droplet dynamics and functions in drosophila melanogaster. In: Methods in Cell Biology. 2013 ; Vol. 116. pp. 53-69.
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