Analysis of cytokine expression in muscle in inflammatory myopathies, Duchenne dystrophy, and non-weak controls

Ingrid Lundberg, Joan M. Brengman, Andrew G Engel

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

We investigated the profiles of cytokine mRNA expression in muscle in 15 cases of inflammatory myopathy (IM) (5 each of polymyositis, inclusion body myositis, and dermatomyositis) and in 10 controls (5 of Duchenne dystrophy and 5 non-weak subjects). Expressions of the predominantly T cell-derived cytokines (interleukin (IL)-2, IL-4, IL-5, and Interferon-γ (IFN-γ), of the predominantly macrophage-derived cytokines (IL-1, IL-6, and tumor necrosis factor-α (TNF-α)), as well as cytokines that can be of either T cell or macrophage origin (granulocyte-macrophage colony stimulating factor (GM-CSF) and transforming growth factor β1 (TGF-β1) and TGF-β2), were monitored by the reverse transcriptase-PCR method. The expression of T cell cytokine mRNAs for IL-2, IL-5, and IFN-γ was generally weak or inconsistent. IL-4 mRNA expression was consistently moderate to strong in polymyositis but generally weak or absent in the other IMs. The expression of macrophage cytokine mRNAs for IL-1 α and IL-1 β was weak or absent in all cases. Variable TNF-α mRNA expression was observed in 12 of 15 IM cases and faint or weak expression in 5 of 10 controls. Very strong GM-CSF.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalJournal of Neuroimmunology
Volume63
Issue number1
DOIs
StatePublished - Dec 1 1995

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Myositis
Cytokines
Muscles
Messenger RNA
Interleukin-1
Polymyositis
Macrophages
Interleukin-5
Granulocyte-Macrophage Colony-Stimulating Factor
T-Lymphocytes
Interleukin-4
Interferons
Interleukin-2
Tumor Necrosis Factor-alpha
Inclusion Body Myositis
Dermatomyositis
Transforming Growth Factors
Reverse Transcriptase Polymerase Chain Reaction
Interleukin-6

Keywords

  • Duchenne dystrophy
  • Inflammatory myopathy

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Analysis of cytokine expression in muscle in inflammatory myopathies, Duchenne dystrophy, and non-weak controls. / Lundberg, Ingrid; Brengman, Joan M.; Engel, Andrew G.

In: Journal of Neuroimmunology, Vol. 63, No. 1, 01.12.1995, p. 9-16.

Research output: Contribution to journalArticle

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