TY - JOUR
T1 - Analysis of collagen profile and orientation in a chronic ruptured extensor pollicis longus tendon
AU - Sasaki, Koichi
AU - Kunamneni, Adinarayana
AU - Sinai, Anthony
AU - Kamineni, Srinath
PY - 2012/12
Y1 - 2012/12
N2 - Purpose: The purpose of this study is to examine the collagen profile and orientation in a ruptured extensor pollicis longus (EPL) tendon, in order to better understand the collagen response to loss of tensile forces. Methods: A 76-year-old male with a history of rheumatoid arthritis required surgical reconstruction of a chronic EPL rupture using EIP tendon transfer; the refreshed tip of the distal EPL tendon, along with the intervening tubular scar and EIP tendon were further analyzed. Picrosirus red (PSR) staining was performed, and the levels of collagen isoform were determined with western blotting. Results: The results obtained from PSR staining under polarized microscopy showed thin, weakly birefringent, green fibers (collagen III-like) and thick, yellow-red, strongly birefringent fibers (collagen I-like). The arrangement was noted to be much less organized and directional in the ruptured tendon compared to the EIP tendon. Western blotting results showed the presence of collagen I, II, III, V, VI and X, with the ratio comparison to collagen I markedly different between a normal EIP tendon and the ruptured EPL tendon. Conclusion: EPL tendon rupture is accompanied by collagen disorganization and significant alteration in the collagen isoform expression, postulated to be a consequence of the loss of tensile forces.
AB - Purpose: The purpose of this study is to examine the collagen profile and orientation in a ruptured extensor pollicis longus (EPL) tendon, in order to better understand the collagen response to loss of tensile forces. Methods: A 76-year-old male with a history of rheumatoid arthritis required surgical reconstruction of a chronic EPL rupture using EIP tendon transfer; the refreshed tip of the distal EPL tendon, along with the intervening tubular scar and EIP tendon were further analyzed. Picrosirus red (PSR) staining was performed, and the levels of collagen isoform were determined with western blotting. Results: The results obtained from PSR staining under polarized microscopy showed thin, weakly birefringent, green fibers (collagen III-like) and thick, yellow-red, strongly birefringent fibers (collagen I-like). The arrangement was noted to be much less organized and directional in the ruptured tendon compared to the EIP tendon. Western blotting results showed the presence of collagen I, II, III, V, VI and X, with the ratio comparison to collagen I markedly different between a normal EIP tendon and the ruptured EPL tendon. Conclusion: EPL tendon rupture is accompanied by collagen disorganization and significant alteration in the collagen isoform expression, postulated to be a consequence of the loss of tensile forces.
KW - Collagen type
KW - Extensor pollicis longus tendon
KW - Picrosirus red staining
KW - Rupture
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U2 - 10.1142/S0218957712720062
DO - 10.1142/S0218957712720062
M3 - Article
AN - SCOPUS:84871304340
SN - 0218-9577
VL - 15
JO - Journal of Musculoskeletal Research
JF - Journal of Musculoskeletal Research
IS - 4
M1 - 1272006
ER -