Analysis of caspase activation during apoptosis.

Scott H Kaufmann, T. J. Kottke, L. M. Martins, A. J. Henzing, W. C. Earnshaw

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This unit describes three methods for the detection of caspase activation as cells undergo apoptosis. Simple and relatively quantitative enzymatic assays are provided using suitable substrates. Because the various low-molecular-weight substrates available for these assays are not selective, however, the assays do not accurately distinguish between various caspases. Immunoblotting is described for following the activation of specific caspases. When coupled with subcellular fractionation, this method can provide large amounts of temporal and spatial information about caspase activation. Finally, affinity labeling protocols are provided for detecting active caspases in whole-cell lysates or subcellular fractions.

Original languageEnglish (US)
JournalCurrent protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]
VolumeChapter 18
StatePublished - Aug 2001

Fingerprint

Activation Analysis
Caspases
Apoptosis
Subcellular Fractions
Enzyme Assays
Immunoblotting
Molecular Weight

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Analysis of caspase activation during apoptosis. / Kaufmann, Scott H; Kottke, T. J.; Martins, L. M.; Henzing, A. J.; Earnshaw, W. C.

In: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.], Vol. Chapter 18, 08.2001.

Research output: Contribution to journalArticle

Kaufmann, Scott H ; Kottke, T. J. ; Martins, L. M. ; Henzing, A. J. ; Earnshaw, W. C. / Analysis of caspase activation during apoptosis. In: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]. 2001 ; Vol. Chapter 18.
@article{6e0073b3dcef40c79390a5af05252aa8,
title = "Analysis of caspase activation during apoptosis.",
abstract = "This unit describes three methods for the detection of caspase activation as cells undergo apoptosis. Simple and relatively quantitative enzymatic assays are provided using suitable substrates. Because the various low-molecular-weight substrates available for these assays are not selective, however, the assays do not accurately distinguish between various caspases. Immunoblotting is described for following the activation of specific caspases. When coupled with subcellular fractionation, this method can provide large amounts of temporal and spatial information about caspase activation. Finally, affinity labeling protocols are provided for detecting active caspases in whole-cell lysates or subcellular fractions.",
author = "Kaufmann, {Scott H} and Kottke, {T. J.} and Martins, {L. M.} and Henzing, {A. J.} and Earnshaw, {W. C.}",
year = "2001",
month = "8",
language = "English (US)",
volume = "Chapter 18",
journal = "Current Protocols in Cell Biology",
issn = "1934-2500",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Analysis of caspase activation during apoptosis.

AU - Kaufmann, Scott H

AU - Kottke, T. J.

AU - Martins, L. M.

AU - Henzing, A. J.

AU - Earnshaw, W. C.

PY - 2001/8

Y1 - 2001/8

N2 - This unit describes three methods for the detection of caspase activation as cells undergo apoptosis. Simple and relatively quantitative enzymatic assays are provided using suitable substrates. Because the various low-molecular-weight substrates available for these assays are not selective, however, the assays do not accurately distinguish between various caspases. Immunoblotting is described for following the activation of specific caspases. When coupled with subcellular fractionation, this method can provide large amounts of temporal and spatial information about caspase activation. Finally, affinity labeling protocols are provided for detecting active caspases in whole-cell lysates or subcellular fractions.

AB - This unit describes three methods for the detection of caspase activation as cells undergo apoptosis. Simple and relatively quantitative enzymatic assays are provided using suitable substrates. Because the various low-molecular-weight substrates available for these assays are not selective, however, the assays do not accurately distinguish between various caspases. Immunoblotting is described for following the activation of specific caspases. When coupled with subcellular fractionation, this method can provide large amounts of temporal and spatial information about caspase activation. Finally, affinity labeling protocols are provided for detecting active caspases in whole-cell lysates or subcellular fractions.

UR - http://www.scopus.com/inward/record.url?scp=39049109212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39049109212&partnerID=8YFLogxK

M3 - Article

VL - Chapter 18

JO - Current Protocols in Cell Biology

JF - Current Protocols in Cell Biology

SN - 1934-2500

ER -