Analysis of autoantibody binding to 52-kd paraneoplastic cerebellar degeneration-associated antigen expressed in recombinant proteins

Koichiro Sakai, Tomoko Ogasawara, Genjiro Hirose, Kurt A. Jaeckle, John E. Greenlee

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A 52-kd neural antigen was reported to be recognized by anti-Purkinje cell antibodies in serum of a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. In this study, we demonstrate that this neural antigen is recognized by antibodies known as anti-Purkinje cell antibody type I (PCAb Type I) and anti-YO. The letter's antigen is reported to be specific for the 62- to 64-kd antigen CDR62. Assuming that the 52-kd and 62-to 64-kd antigens share a common epitope(s) recognized by all of these antibodies, we examined the antigenic region on the 52-kd protein by immunoblots with deletion fragment proteins of the recombinant 52-kd protein. A major epitope was localized in the region of amino acid residues 94 to 133 of the 52-kd protein, which is the site of a leucine zipper motif. The potential pathogenicity of PCAb Type I is discussed.

Original languageEnglish (US)
Pages (from-to)373-380
Number of pages8
JournalAnnals of Neurology
Volume33
Issue number4
StatePublished - Apr 1993
Externally publishedYes

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Paraneoplastic Cerebellar Degeneration
Recombinant Proteins
Autoantibodies
Purkinje Cells
Antigens
Antibodies
Epitopes
Leucine Zippers
Proteins
Virulence
Anti-Idiotypic Antibodies
Carcinoma
Amino Acids
Serum

ASJC Scopus subject areas

  • Neuroscience(all)

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Analysis of autoantibody binding to 52-kd paraneoplastic cerebellar degeneration-associated antigen expressed in recombinant proteins. / Sakai, Koichiro; Ogasawara, Tomoko; Hirose, Genjiro; Jaeckle, Kurt A.; Greenlee, John E.

In: Annals of Neurology, Vol. 33, No. 4, 04.1993, p. 373-380.

Research output: Contribution to journalArticle

Sakai, K, Ogasawara, T, Hirose, G, Jaeckle, KA & Greenlee, JE 1993, 'Analysis of autoantibody binding to 52-kd paraneoplastic cerebellar degeneration-associated antigen expressed in recombinant proteins', Annals of Neurology, vol. 33, no. 4, pp. 373-380.
Sakai, Koichiro ; Ogasawara, Tomoko ; Hirose, Genjiro ; Jaeckle, Kurt A. ; Greenlee, John E. / Analysis of autoantibody binding to 52-kd paraneoplastic cerebellar degeneration-associated antigen expressed in recombinant proteins. In: Annals of Neurology. 1993 ; Vol. 33, No. 4. pp. 373-380.
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