Analysis of Amyloid in Medullary Thyroid Carcinoma by Mass Spectrometry-Based Proteomic Analysis

Lori A. Erickson, Julie A. Vrana, Jason Theis, Michael Rivera, Ricardo V. Lloyd, Ellen McPhail, Jun Zhang

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Amyloid is a characteristic histologic feature in medullary thyroid carcinomas (MTC). We utilized a novel mass spectrometry-based proteomic analysis to determine if we could identify specific proteins associated with amyloid in MTC. We studied 9 MTC (1 multiple endocrine neoplasia type 2A, 1 familial MTC, and 7 sporadic). Laser microdissection was utilized to sample the amyloid which was then trypsin digested and evaluated by liquid chromatography electrospray tandem MS (LC-MS/MS) which identified the presence of amyloidogenic proteins in all cases of MTC. High levels of calcitonin were identified in all 9 cases of MTC. Secretogranin-1 was identified in 6 of 9 MTC. Calcitonin gene-related peptide was identified in 4 of 9 cases of MTC. LC-MS/MS proteomic analysis provides a rapid, highly specific, and sensitive method for identification of the specific type of amyloid in these endocrine tumors. This approach may allow classification of different forms of endocrine amyloid present in neuroendocrine tumors.

Original languageEnglish (US)
Pages (from-to)291-295
Number of pages5
JournalEndocrine Pathology
Volume26
Issue number4
DOIs
StatePublished - Dec 1 2015

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Keywords

  • Amyloid
  • Calcitonin
  • Endocrine
  • Medullary thyroid carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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