Analysis of a novel mechanism of neuronal toxicity produced by an apolipoprotein E-derived peptide

Krista L. Moulder, Masaaki Narita, Louis K. Chang, Guojun D Bu, Eugene M. Johnson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The apolipoprotein E (apoE)-derived peptide (141-155)2 has a neurotoxic effect, implying that apoE itself could be a source of toxicity in Alzheimer's disease brain. We characterized the toxicity of this peptide on superior cervical ganglion (SCG) neurons and compared the death with the apoptotic death that occurs after nerve growth factor (NGF) deprivation in these cells. A dose of 10 μM apoE (141-155)2 resulted in the death of ~50% of the neurons within 24 h. Nuclear condensation and DNA fragmentation preceded the death. However, most inhibitors of NGF deprivation-induced death, including the caspase inhibitor Boc-aspartyl(O-methyl)fluoromethyl ketone and genetic deletion of bax(-/-), had no effect on the toxicity. Inclusion of depolarizing levels of potassium did block the toxicity. Receptor-associated peptide (RAP), an antagonist for apoE receptors, did not protect cells in either SCG or hippocampal cultures. In addition, RAP had no effect on internalization of the apoE peptide. These data support the observation that apoE (141-155)2 is neurotoxic but suggest that the neurotoxicity is distinct from classical apoptosis or necrosis. Furthermore, these results indicate that the toxic effect may occur independently of members of the low-density lipoprotein receptor gene family.

Original languageEnglish (US)
Pages (from-to)1069-1080
Number of pages12
JournalJournal of Neurochemistry
Volume72
Issue number3
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Apolipoproteins E
Toxicity
Peptides
Superior Cervical Ganglion
Peptide Receptors
Nerve Growth Factor
Neurons
Low Density Lipoprotein Receptor-Related Protein-1
Caspase Inhibitors
LDL Receptors
Poisons
DNA Fragmentation
Ketones
Condensation
Brain
Potassium
Alzheimer Disease
Necrosis
Genes
Apoptosis

Keywords

  • Alzheimer's disease
  • Apolipoprotein E
  • Apoptosis
  • Low- density lipoprotein receptor gene family
  • Neurotoxicity
  • Sympathetic neurons

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Analysis of a novel mechanism of neuronal toxicity produced by an apolipoprotein E-derived peptide. / Moulder, Krista L.; Narita, Masaaki; Chang, Louis K.; Bu, Guojun D; Johnson, Eugene M.

In: Journal of Neurochemistry, Vol. 72, No. 3, 1999, p. 1069-1080.

Research output: Contribution to journalArticle

Moulder, Krista L. ; Narita, Masaaki ; Chang, Louis K. ; Bu, Guojun D ; Johnson, Eugene M. / Analysis of a novel mechanism of neuronal toxicity produced by an apolipoprotein E-derived peptide. In: Journal of Neurochemistry. 1999 ; Vol. 72, No. 3. pp. 1069-1080.
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