Analysis and location of a rice BAC clone containing telomeric DNA sequences

Wenxue Zhai, Hao Chen, Huihuang Yan, Changjie Yan, Guoliang Wang, Lihuang Zhu

Research output: Contribution to journalArticle

Abstract

BAC2, a rice BAC clone containing (TTTAGGG)n homologous sequences, was analyzed by Southern hybridization and DNA sequencing of its subclones. It was disclosed that there were many tandem repeated satellite DNA sequences, called TA352, as well as simple tandem repeats consisting of TTTAGGG or its variant within the BAC2 insert. A 0.8 kb (TTTAGGG)n-containing fragment in BAC2 was mapped in the telomere regions of at least 5 pairs of rice chromosomes by using fluorescence in situ hybridization (FISH). By RFLP analysis of low copy sequences the BAC2 clone was localized in one terminal region of chromosome 6. All the results strongly suggest that the telomeric DNA sequences of rice are TTTAGGG or its variant, and the linked satellite DNA TA352 sequences belong to telomere-associated sequences.

Original languageEnglish (US)
Pages (from-to)68-73
Number of pages6
JournalScience in China, Series C: Life Sciences
Volume42
Issue number1
StatePublished - Jan 1 1999

Keywords

  • BAC clone
  • Rice
  • Telomere-associated sequences
  • Telomeric sequences

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Analysis and location of a rice BAC clone containing telomeric DNA sequences'. Together they form a unique fingerprint.

Cite this