An overview on the isolation and analysis of circulating tumor DNA in plasma and serum

Jin Jen, Li Wu, David Sidransky

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Molecular biology has brought about a new dimension to our understanding and analysis of human cancer. Diagnosis and prognosis of cancer have increasingly benefited from the use of molecular markers and molecular approaches. Although the description of circulating tumor DNA in the blood has a long history, the clinical implications of its presence require a large body of well-controlled and stringently performed large research and clinical tests. This chapter has provided an overview of the essential elements for the preparation and analysis of circulating DNA in the plasma or serum. We hope that the basic approaches and tips summarized here will serve as a foundation for the rigorous and comprehensive molecular analysis of circulating nucleic acids in the years to come.

Original languageEnglish (US)
Pages (from-to)8-12
Number of pages5
JournalAnnals of the New York Academy of Sciences
Volume906
StatePublished - 2000
Externally publishedYes

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Tumors
Plasmas
Molecular biology
DNA
Serum
Nucleic Acids
Neoplasms
Blood
Molecular Biology
History
Research
Isolation
Plasma
Cancer
Controlled

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

An overview on the isolation and analysis of circulating tumor DNA in plasma and serum. / Jen, Jin; Wu, Li; Sidransky, David.

In: Annals of the New York Academy of Sciences, Vol. 906, 2000, p. 8-12.

Research output: Contribution to journalArticle

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