Methylamine-induced cells of Pseudomonas MA (ATCC No. 23819) contain a particulate enzyme system which catalyzes the reaction: 2 N-methyl l-glutamate + O2 → 2 l-glutamate + 2 formaldehyde + (H2O). The stoichiometry of this reaction (2 moles N-methyl l-glutamate utilized per mole of oxygen) suggests that the enzyme is a dehydrogenase. During the oxidation of N-methyl l-glutamate, cytochromes b and c are enzymatically reduced, supporting the conclusion that the enzyme is a respiratory chain-linked dehydrogenase. It is proposed that the function of this enzyme is to activate the methyl group of N-methyl l-glutamate so that it may be further metabolized by this microorganism.
ASJC Scopus subject areas
- Molecular Biology