An Improved, Chemically Modified RNA Encoding BMP-2 Enhances Osteogenesis in Vitro and in Vivo

Wen Zhang, Rodolfo E. De La Vega, Michael J. Coenen, Sebastian A. Müller, Carlos J. Peniche Silva, Manish K. Aneja, Christian Plank, Martijn Van Griensven, Christopher H Evans, Elizabeth R. Balmayor

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The first therapeutic application of messenger RNA (mRNA) was suggested more than two decades ago. However, its application was constrained by the ability of mRNA to activate the innate immune response, cytotoxicity, and poor potency. We and others recently demonstrated that these undesirable properties of mRNA may be overcome by alterating its structure. In this study, we developed a new chemically modified mRNA coding for BMP-2 with improved osteogenic features. To develop this new construct, we removed from the mRNA sequence the following undesirable elements: an upstream open reading frame in the 5′-untranslated region (UTR) and a polyadenylation element together with an AU-rich tract in the 3′UTR. In addition, a translation initiator of short UTRs (TISU) was introduced together with 5-iodo modified pyrimidine nucleotides. The new TISU BMP-2 chemically modified RNA (cmRNA) showed robust BMP-2 production in vitro in cell lines (HEK293 and MC3T3) and primary cells (muscle-derived mesenchymal stem cells). Stem cells additionally showed upregulation of osteogenic and angiogenic genes as a result of the TISU BMP-2 cmRNA transfection. The in vivo osteogenic properties of TISU BMP-2 cmRNA were explored in a critical-sized femoral defect in the rat. For this, the TISU BMP-2 cmRNA was loaded into collagen sponges to form transcript-activated matrices. Animals treated with TISU BMP-2 cmRNA showed superior bone formation that seemed to recapitulate endochondral ossification. The higher of the two doses examined in this model showed more robust new tissue formation. Finally, improved vascularization was detected in the healing area for animals treated with TISU BMP-2 cmRNA. The use of chemically modified RNA (cmRNA) with increased stability using translation initiator of short untranslated regions (TISU) offers the prospect of finally allowing us to unlock the potent osteogenic properties of BMP-2 in a clinically expedient manner. As noted, delivery of recombinant BMP-2 protein has had modest clinical efficacy, whereas gene delivery is effective but very difficult to translate into human clinical use. This study shows the great potential of cmRNA encoding BMP-2 with TISU in a long-bone critical-sized rat model.

Original languageEnglish (US)
Pages (from-to)131-144
Number of pages14
JournalTissue Engineering - Part A
Volume25
Issue number1-2
DOIs
StatePublished - Jan 1 2019

Fingerprint

Untranslated Regions
RNA
Osteogenesis
Messenger RNA
Stem cells
Rats
Bone
Animals
Genes
Pyrimidine Nucleotides
In Vitro Techniques
Polyadenylation
Cytotoxicity
Nucleotides
5' Untranslated Regions
Collagen
Porifera
Thigh
Mesenchymal Stromal Cells
Muscle

Keywords

  • BMP-2
  • bone healing
  • modified mRNA
  • mRNA
  • transcript therapy
  • vascularization

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Zhang, W., De La Vega, R. E., Coenen, M. J., Müller, S. A., Peniche Silva, C. J., Aneja, M. K., ... Balmayor, E. R. (2019). An Improved, Chemically Modified RNA Encoding BMP-2 Enhances Osteogenesis in Vitro and in Vivo. Tissue Engineering - Part A, 25(1-2), 131-144. https://doi.org/10.1089/ten.tea.2018.0112

An Improved, Chemically Modified RNA Encoding BMP-2 Enhances Osteogenesis in Vitro and in Vivo. / Zhang, Wen; De La Vega, Rodolfo E.; Coenen, Michael J.; Müller, Sebastian A.; Peniche Silva, Carlos J.; Aneja, Manish K.; Plank, Christian; Van Griensven, Martijn; Evans, Christopher H; Balmayor, Elizabeth R.

In: Tissue Engineering - Part A, Vol. 25, No. 1-2, 01.01.2019, p. 131-144.

Research output: Contribution to journalArticle

Zhang, W, De La Vega, RE, Coenen, MJ, Müller, SA, Peniche Silva, CJ, Aneja, MK, Plank, C, Van Griensven, M, Evans, CH & Balmayor, ER 2019, 'An Improved, Chemically Modified RNA Encoding BMP-2 Enhances Osteogenesis in Vitro and in Vivo', Tissue Engineering - Part A, vol. 25, no. 1-2, pp. 131-144. https://doi.org/10.1089/ten.tea.2018.0112
Zhang W, De La Vega RE, Coenen MJ, Müller SA, Peniche Silva CJ, Aneja MK et al. An Improved, Chemically Modified RNA Encoding BMP-2 Enhances Osteogenesis in Vitro and in Vivo. Tissue Engineering - Part A. 2019 Jan 1;25(1-2):131-144. https://doi.org/10.1089/ten.tea.2018.0112
Zhang, Wen ; De La Vega, Rodolfo E. ; Coenen, Michael J. ; Müller, Sebastian A. ; Peniche Silva, Carlos J. ; Aneja, Manish K. ; Plank, Christian ; Van Griensven, Martijn ; Evans, Christopher H ; Balmayor, Elizabeth R. / An Improved, Chemically Modified RNA Encoding BMP-2 Enhances Osteogenesis in Vitro and in Vivo. In: Tissue Engineering - Part A. 2019 ; Vol. 25, No. 1-2. pp. 131-144.
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