An Examination of the Association between FOXA1 Staining Level and Biochemical Recurrence following Salvage Radiation Therapy for Recurrent Prostate Cancer

Michael G. Heckman, Jessica L. Robinson, Katherine S. Tzou, Alexander Parker, Kevin J. Wu, Tracy W. Hilton, William J. Howat, Jodi L. Miller, Pamela A. Kreinest, Thomas M. Pisansky, Steven E. Schild, Jennifer L. Peterson, Laura A. Vallow, Jason S. Carroll, Steven J. Buskirk

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

BACKGROUND: Standardly collected clinical and pathological patient information has demonstrated only moderate ability to predict risk of biochemical recurrence (BCR) of prostate cancer in men undergoing salvage radiation therapy (SRT) for a rising PSA after radical prostatectomy (RP). Although elevated FOXA1 staining has been associated with poor patient outcomes following RP, it has not been studied in the specific setting of SRT after RP. The aim of this study was to evaluate the association between FOXA1 staining level and BCR after SRT for recurrent prostate cancer.

METHODS: A total of 141 men who underwent SRT at our institution were included. FOXA1 staining levels in primary tumor samples were detected using immunohistochemistry. FOXA1 staining percentage and intensity were measured and multiplied together to obtain a FOXA1 H-score (range 0-12) which was our primary staining measure. P-values ≤ 0.0056 were considered as statistically significant after applying a Bonferroni correction for multiple comparisons.

RESULTS: There was not a significant association between FOXA1 H-score and risk of BCR when considering H-score as an ordinal variable or as a categorical variable (all P ≥ 0.090). Similarly, no significant associations with BCR were observed for FOXA1 staining percentage or staining intensity (all P ≥ 0.14).

CONCLUSIONS: FOXA1 staining level does not appear to have a major impact on risk of BCR after SRT.

Original languageEnglish (US)
Pages (from-to)e0151785
JournalPLoS One
Volume11
Issue number3
DOIs
StatePublished - 2016

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Salvaging
Salvage Therapy
Radiotherapy
prostatic neoplasms
radiotherapy
Prostatic Neoplasms
Staining and Labeling
Recurrence
Prostatectomy
Tumors
staining
immunohistochemistry
Immunohistochemistry
neoplasms

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

An Examination of the Association between FOXA1 Staining Level and Biochemical Recurrence following Salvage Radiation Therapy for Recurrent Prostate Cancer. / Heckman, Michael G.; Robinson, Jessica L.; Tzou, Katherine S.; Parker, Alexander; Wu, Kevin J.; Hilton, Tracy W.; Howat, William J.; Miller, Jodi L.; Kreinest, Pamela A.; Pisansky, Thomas M.; Schild, Steven E.; Peterson, Jennifer L.; Vallow, Laura A.; Carroll, Jason S.; Buskirk, Steven J.

In: PLoS One, Vol. 11, No. 3, 2016, p. e0151785.

Research output: Contribution to journalArticle

Heckman, MG, Robinson, JL, Tzou, KS, Parker, A, Wu, KJ, Hilton, TW, Howat, WJ, Miller, JL, Kreinest, PA, Pisansky, TM, Schild, SE, Peterson, JL, Vallow, LA, Carroll, JS & Buskirk, SJ 2016, 'An Examination of the Association between FOXA1 Staining Level and Biochemical Recurrence following Salvage Radiation Therapy for Recurrent Prostate Cancer', PLoS One, vol. 11, no. 3, pp. e0151785. https://doi.org/10.1371/journal.pone.0151785
Heckman, Michael G. ; Robinson, Jessica L. ; Tzou, Katherine S. ; Parker, Alexander ; Wu, Kevin J. ; Hilton, Tracy W. ; Howat, William J. ; Miller, Jodi L. ; Kreinest, Pamela A. ; Pisansky, Thomas M. ; Schild, Steven E. ; Peterson, Jennifer L. ; Vallow, Laura A. ; Carroll, Jason S. ; Buskirk, Steven J. / An Examination of the Association between FOXA1 Staining Level and Biochemical Recurrence following Salvage Radiation Therapy for Recurrent Prostate Cancer. In: PLoS One. 2016 ; Vol. 11, No. 3. pp. e0151785.
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abstract = "BACKGROUND: Standardly collected clinical and pathological patient information has demonstrated only moderate ability to predict risk of biochemical recurrence (BCR) of prostate cancer in men undergoing salvage radiation therapy (SRT) for a rising PSA after radical prostatectomy (RP). Although elevated FOXA1 staining has been associated with poor patient outcomes following RP, it has not been studied in the specific setting of SRT after RP. The aim of this study was to evaluate the association between FOXA1 staining level and BCR after SRT for recurrent prostate cancer.METHODS: A total of 141 men who underwent SRT at our institution were included. FOXA1 staining levels in primary tumor samples were detected using immunohistochemistry. FOXA1 staining percentage and intensity were measured and multiplied together to obtain a FOXA1 H-score (range 0-12) which was our primary staining measure. P-values ≤ 0.0056 were considered as statistically significant after applying a Bonferroni correction for multiple comparisons.RESULTS: There was not a significant association between FOXA1 H-score and risk of BCR when considering H-score as an ordinal variable or as a categorical variable (all P ≥ 0.090). Similarly, no significant associations with BCR were observed for FOXA1 staining percentage or staining intensity (all P ≥ 0.14).CONCLUSIONS: FOXA1 staining level does not appear to have a major impact on risk of BCR after SRT.",
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AU - Heckman, Michael G.

AU - Robinson, Jessica L.

AU - Tzou, Katherine S.

AU - Parker, Alexander

AU - Wu, Kevin J.

AU - Hilton, Tracy W.

AU - Howat, William J.

AU - Miller, Jodi L.

AU - Kreinest, Pamela A.

AU - Pisansky, Thomas M.

AU - Schild, Steven E.

AU - Peterson, Jennifer L.

AU - Vallow, Laura A.

AU - Carroll, Jason S.

AU - Buskirk, Steven J.

PY - 2016

Y1 - 2016

N2 - BACKGROUND: Standardly collected clinical and pathological patient information has demonstrated only moderate ability to predict risk of biochemical recurrence (BCR) of prostate cancer in men undergoing salvage radiation therapy (SRT) for a rising PSA after radical prostatectomy (RP). Although elevated FOXA1 staining has been associated with poor patient outcomes following RP, it has not been studied in the specific setting of SRT after RP. The aim of this study was to evaluate the association between FOXA1 staining level and BCR after SRT for recurrent prostate cancer.METHODS: A total of 141 men who underwent SRT at our institution were included. FOXA1 staining levels in primary tumor samples were detected using immunohistochemistry. FOXA1 staining percentage and intensity were measured and multiplied together to obtain a FOXA1 H-score (range 0-12) which was our primary staining measure. P-values ≤ 0.0056 were considered as statistically significant after applying a Bonferroni correction for multiple comparisons.RESULTS: There was not a significant association between FOXA1 H-score and risk of BCR when considering H-score as an ordinal variable or as a categorical variable (all P ≥ 0.090). Similarly, no significant associations with BCR were observed for FOXA1 staining percentage or staining intensity (all P ≥ 0.14).CONCLUSIONS: FOXA1 staining level does not appear to have a major impact on risk of BCR after SRT.

AB - BACKGROUND: Standardly collected clinical and pathological patient information has demonstrated only moderate ability to predict risk of biochemical recurrence (BCR) of prostate cancer in men undergoing salvage radiation therapy (SRT) for a rising PSA after radical prostatectomy (RP). Although elevated FOXA1 staining has been associated with poor patient outcomes following RP, it has not been studied in the specific setting of SRT after RP. The aim of this study was to evaluate the association between FOXA1 staining level and BCR after SRT for recurrent prostate cancer.METHODS: A total of 141 men who underwent SRT at our institution were included. FOXA1 staining levels in primary tumor samples were detected using immunohistochemistry. FOXA1 staining percentage and intensity were measured and multiplied together to obtain a FOXA1 H-score (range 0-12) which was our primary staining measure. P-values ≤ 0.0056 were considered as statistically significant after applying a Bonferroni correction for multiple comparisons.RESULTS: There was not a significant association between FOXA1 H-score and risk of BCR when considering H-score as an ordinal variable or as a categorical variable (all P ≥ 0.090). Similarly, no significant associations with BCR were observed for FOXA1 staining percentage or staining intensity (all P ≥ 0.14).CONCLUSIONS: FOXA1 staining level does not appear to have a major impact on risk of BCR after SRT.

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