An evaluation of fluorometric proteinase assays which employ fluorescamine

Christopher H. Evans, John D. Ridella

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The sensitivity and utility of proteinase assays employing fluorescamine, a compound which reacts with primary amines to form a fluorescent adduct, was assessed. As little as 1 ng of purified trypsin or clostridiopeptidase A could be detected within 3 h of incubation at 37°C, using casein or gelatin as substrates. Increasing the incubation period to 18 h permitted the detection of 250 pg of each enzyme. When gelled collagen was utilized as substrate, the sensitivity to clostridiopeptidase A was reduced to 2.5 ng at 3 h and 500 pg at 18 h. The techniques could be used to measure the gelatinase, caseinase, and collagenase activities of culture media conditioned by synovial tissue. The main disadvantage of this assay is its susceptibility to interference by compounds which fluoresce or quench. This, in turn, necessitates additional blanks, which may render the assay tedious.

Original languageEnglish (US)
Pages (from-to)411-420
Number of pages10
JournalAnalytical Biochemistry
Volume142
Issue number2
DOIs
StatePublished - Nov 1 1984

Keywords

  • collagenase
  • fluorescence
  • proteases
  • synovial cell culture
  • trypsin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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