Abstract
Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4527-4532 |
| Number of pages | 6 |
| Journal | Analytical Methods |
| Volume | 9 |
| Issue number | 31 |
| DOIs | |
| State | Published - Aug 21 2017 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Chemical Engineering(all)
- Engineering(all)
Cite this
An electrochemical aptasensor for detection of bovine interferon gamma. / Crulhas, Bruno P.; Hadley, Dustin; Liu, Ying; Shin, Dong Sik; Stybayeva, Gulnaz; Imanbekova, Meruyert; Hill, Ashley E.; Pedrosa, Valber; Revzin, Alexander.
In: Analytical Methods, Vol. 9, No. 31, 21.08.2017, p. 4527-4532.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An electrochemical aptasensor for detection of bovine interferon gamma
AU - Crulhas, Bruno P.
AU - Hadley, Dustin
AU - Liu, Ying
AU - Shin, Dong Sik
AU - Stybayeva, Gulnaz
AU - Imanbekova, Meruyert
AU - Hill, Ashley E.
AU - Pedrosa, Valber
AU - Revzin, Alexander
PY - 2017/8/21
Y1 - 2017/8/21
N2 - Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.
AB - Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.
UR - http://www.scopus.com/inward/record.url?scp=85027335841&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027335841&partnerID=8YFLogxK
U2 - 10.1039/c7ay01313b
DO - 10.1039/c7ay01313b
M3 - Article
AN - SCOPUS:85027335841
VL - 9
SP - 4527
EP - 4532
JO - Analytical Methods
JF - Analytical Methods
SN - 1759-9660
IS - 31
ER -