An electrochemical aptasensor for detection of bovine interferon gamma

Bruno P. Crulhas; Dustin Hadley; Ying Liu; Dong Sik Shin; Gulnaz Stybayeva; Meruyert Imanbekova; Ashley E. Hill; Valber Pedrosa; Alexander Revzin

doi:10.1039/c7ay01313b

An electrochemical aptasensor for detection of bovine interferon gamma

Bruno P. Crulhas, Dustin Hadley, Ying Liu, Dong Sik Shin, Gulnaz Stybayeva, Meruyert Imanbekova, Ashley E. Hill, Valber Pedrosa, Alexander Revzin

Physiology & Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.

Original language	English (US)
Pages (from-to)	4527-4532
Number of pages	6
Journal	Analytical Methods
Volume	9
Issue number	31
DOIs	https://doi.org/10.1039/c7ay01313b
State	Published - Aug 21 2017

ASJC Scopus subject areas

Analytical Chemistry
General Chemical Engineering
General Engineering

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title = "An electrochemical aptasensor for detection of bovine interferon gamma",

abstract = "Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.",

author = "Crulhas, {Bruno P.} and Dustin Hadley and Ying Liu and Shin, {Dong Sik} and Gulnaz Stybayeva and Meruyert Imanbekova and Hill, {Ashley E.} and Valber Pedrosa and Alexander Revzin",

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doi = "10.1039/c7ay01313b",

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T1 - An electrochemical aptasensor for detection of bovine interferon gamma

AU - Crulhas, Bruno P.

AU - Hadley, Dustin

AU - Liu, Ying

AU - Shin, Dong Sik

AU - Stybayeva, Gulnaz

AU - Imanbekova, Meruyert

AU - Hill, Ashley E.

AU - Pedrosa, Valber

AU - Revzin, Alexander

PY - 2017/8/21

Y1 - 2017/8/21

N2 - Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.

AB - Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.

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An electrochemical aptasensor for detection of bovine interferon gamma

Abstract

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