An electrochemical aptasensor for detection of bovine interferon gamma

Bruno P. Crulhas, Dustin Hadley, Ying Liu, Dong Sik Shin, Gulnaz Stybayeva, Meruyert Imanbekova, Ashley E. Hill, Valber Pedrosa, Alexander Revzin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.

Original languageEnglish (US)
Pages (from-to)4527-4532
Number of pages6
JournalAnalytical Methods
Volume9
Issue number31
DOIs
StatePublished - Aug 21 2017

Fingerprint

Interferons
Interferon-gamma
Blood
Electrodes
T-cells
Methylene Blue
Voltammetry
Biosensors
Gold
Self assembly
Buffers
Electrons
Testing
Oxidation-Reduction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

Cite this

Crulhas, B. P., Hadley, D., Liu, Y., Shin, D. S., Stybayeva, G., Imanbekova, M., ... Revzin, A. (2017). An electrochemical aptasensor for detection of bovine interferon gamma. Analytical Methods, 9(31), 4527-4532. https://doi.org/10.1039/c7ay01313b

An electrochemical aptasensor for detection of bovine interferon gamma. / Crulhas, Bruno P.; Hadley, Dustin; Liu, Ying; Shin, Dong Sik; Stybayeva, Gulnaz; Imanbekova, Meruyert; Hill, Ashley E.; Pedrosa, Valber; Revzin, Alexander.

In: Analytical Methods, Vol. 9, No. 31, 21.08.2017, p. 4527-4532.

Research output: Contribution to journalArticle

Crulhas, BP, Hadley, D, Liu, Y, Shin, DS, Stybayeva, G, Imanbekova, M, Hill, AE, Pedrosa, V & Revzin, A 2017, 'An electrochemical aptasensor for detection of bovine interferon gamma', Analytical Methods, vol. 9, no. 31, pp. 4527-4532. https://doi.org/10.1039/c7ay01313b
Crulhas BP, Hadley D, Liu Y, Shin DS, Stybayeva G, Imanbekova M et al. An electrochemical aptasensor for detection of bovine interferon gamma. Analytical Methods. 2017 Aug 21;9(31):4527-4532. https://doi.org/10.1039/c7ay01313b
Crulhas, Bruno P. ; Hadley, Dustin ; Liu, Ying ; Shin, Dong Sik ; Stybayeva, Gulnaz ; Imanbekova, Meruyert ; Hill, Ashley E. ; Pedrosa, Valber ; Revzin, Alexander. / An electrochemical aptasensor for detection of bovine interferon gamma. In: Analytical Methods. 2017 ; Vol. 9, No. 31. pp. 4527-4532.
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