Amplification and fusion of long fragments of hepatitis C virus genome

Lian Fu Wang, Marek Radkowski, Hugo Vargas, Jorge Rakela, Tomasz Laskus

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The 'long PCR' was used for amplification of hepatitis C virus (HCV) subgenomic fragments from liver. After testing several commercially available systems, it was found that Tth as the major enzyme is superior to using Tag. Employing a mixture of Tth and Vent polymerase (rTth polymerase, XL, Perkin Elmer) it was possible to amplify 4.6-kb and 9-kb fragments from biological samples containing as little as 102 and 104 viral copies, respectively. It was also demonstrated that 'long PCR' is useful for joining together large size amplification products.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalJournal of Virological Methods
Volume68
Issue number2
DOIs
StatePublished - Nov 1 1997

Keywords

  • Amplification
  • Fusion
  • HCV
  • Hepatitis C virus
  • Long PCR

ASJC Scopus subject areas

  • Virology

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