Amplification and fusion of long fragments of hepatitis C virus genome

Lian Fu Wang, Marek Radkowski, Hugo E Vargas, Jorge Rakela, Tomasz Laskus

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The 'long PCR' was used for amplification of hepatitis C virus (HCV) subgenomic fragments from liver. After testing several commercially available systems, it was found that Tth as the major enzyme is superior to using Tag. Employing a mixture of Tth and Vent polymerase (rTth polymerase, XL, Perkin Elmer) it was possible to amplify 4.6-kb and 9-kb fragments from biological samples containing as little as 102 and 104 viral copies, respectively. It was also demonstrated that 'long PCR' is useful for joining together large size amplification products.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalJournal of Virological Methods
Volume68
Issue number2
DOIs
StatePublished - Nov 1997
Externally publishedYes

Fingerprint

Hepacivirus
Genome
Polymerase Chain Reaction
Liver
Enzymes
Tth polymerase

Keywords

  • Amplification
  • Fusion
  • HCV
  • Hepatitis C virus
  • Long PCR

ASJC Scopus subject areas

  • Virology

Cite this

Amplification and fusion of long fragments of hepatitis C virus genome. / Wang, Lian Fu; Radkowski, Marek; Vargas, Hugo E; Rakela, Jorge; Laskus, Tomasz.

In: Journal of Virological Methods, Vol. 68, No. 2, 11.1997, p. 217-223.

Research output: Contribution to journalArticle

Wang, Lian Fu ; Radkowski, Marek ; Vargas, Hugo E ; Rakela, Jorge ; Laskus, Tomasz. / Amplification and fusion of long fragments of hepatitis C virus genome. In: Journal of Virological Methods. 1997 ; Vol. 68, No. 2. pp. 217-223.
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