Amplification and fusion of long fragments of hepatitis C virus genome

Lian Fu Wang; Marek Radkowski; Hugo Vargas; Jorge Rakela; Tomasz Laskus

doi:10.1016/S0166-0934(97)00132-8

Amplification and fusion of long fragments of hepatitis C virus genome

Lian Fu Wang, Marek Radkowski, Hugo Vargas, Jorge Rakela, Tomasz Laskus

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The 'long PCR' was used for amplification of hepatitis C virus (HCV) subgenomic fragments from liver. After testing several commercially available systems, it was found that Tth as the major enzyme is superior to using Tag. Employing a mixture of Tth and Vent polymerase (rTth polymerase, XL, Perkin Elmer) it was possible to amplify 4.6-kb and 9-kb fragments from biological samples containing as little as 10² and 10⁴ viral copies, respectively. It was also demonstrated that 'long PCR' is useful for joining together large size amplification products.

Original language	English (US)
Pages (from-to)	217-223
Number of pages	7
Journal	Journal of Virological Methods
Volume	68
Issue number	2
DOIs	https://doi.org/10.1016/S0166-0934(97)00132-8
State	Published - Nov 1997

Keywords

Amplification
Fusion
HCV
Hepatitis C virus
Long PCR

ASJC Scopus subject areas

Virology

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10.1016/S0166-0934(97)00132-8

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title = "Amplification and fusion of long fragments of hepatitis C virus genome",

abstract = "The 'long PCR' was used for amplification of hepatitis C virus (HCV) subgenomic fragments from liver. After testing several commercially available systems, it was found that Tth as the major enzyme is superior to using Tag. Employing a mixture of Tth and Vent polymerase (rTth polymerase, XL, Perkin Elmer) it was possible to amplify 4.6-kb and 9-kb fragments from biological samples containing as little as 102 and 104 viral copies, respectively. It was also demonstrated that 'long PCR' is useful for joining together large size amplification products.",

keywords = "Amplification, Fusion, HCV, Hepatitis C virus, Long PCR",

author = "Wang, {Lian Fu} and Marek Radkowski and Hugo Vargas and Jorge Rakela and Tomasz Laskus",

year = "1997",

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T1 - Amplification and fusion of long fragments of hepatitis C virus genome

AU - Wang, Lian Fu

AU - Radkowski, Marek

AU - Vargas, Hugo

AU - Rakela, Jorge

AU - Laskus, Tomasz

PY - 1997/11

Y1 - 1997/11

N2 - The 'long PCR' was used for amplification of hepatitis C virus (HCV) subgenomic fragments from liver. After testing several commercially available systems, it was found that Tth as the major enzyme is superior to using Tag. Employing a mixture of Tth and Vent polymerase (rTth polymerase, XL, Perkin Elmer) it was possible to amplify 4.6-kb and 9-kb fragments from biological samples containing as little as 102 and 104 viral copies, respectively. It was also demonstrated that 'long PCR' is useful for joining together large size amplification products.

AB - The 'long PCR' was used for amplification of hepatitis C virus (HCV) subgenomic fragments from liver. After testing several commercially available systems, it was found that Tth as the major enzyme is superior to using Tag. Employing a mixture of Tth and Vent polymerase (rTth polymerase, XL, Perkin Elmer) it was possible to amplify 4.6-kb and 9-kb fragments from biological samples containing as little as 102 and 104 viral copies, respectively. It was also demonstrated that 'long PCR' is useful for joining together large size amplification products.

KW - Amplification

KW - Fusion

KW - HCV

KW - Hepatitis C virus

KW - Long PCR

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Amplification and fusion of long fragments of hepatitis C virus genome

Abstract

Keywords

ASJC Scopus subject areas

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