AMPK regulates histone H2B O-GlcNAcylation

Qiuran Xu, Caihong Yang, Yu Du, Yali Chen, Hailong Liu, Min Deng, Haoxing Zhang, Lei Zhang, Tongzheng Liu, Qingguang Liu, Liewei M Wang, Zhenkun Lou, Huadong Pei

Research output: Contribution to journalArticle

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Abstract

Histone H2B O-GlcNAcylation is an important post-translational modification of chromatin during gene transcription. However, how this epigenetic modification is regulated remains unclear. Here we found that the energy-sensing adenosine-monophosphate-activated protein kinase (AMPK) could suppress histone H2B O-GlcNAcylation. AMPK directly phosphorylates O-linked -N-acetylglucosamine (O-GlcNAc) transferase (OGT). Although this phosphorylation does not regulate the enzymatic activity of OGT, it inhibits OGT-chromatin association, histone O-GlcNAcylation and gene transcription. Conversely, OGT also O-GlcNAcylates AMPK and positively regulates AMPK activity, creating a feedback loop. Taken together, these results reveal a crosstalk between the LKB1-AMPK and the hexosamine biosynthesis (HBP)-OGT pathways, which coordinate together for the sensing of nutrient state and regulation of gene transcription.

Original languageEnglish (US)
Pages (from-to)5594-5604
Number of pages11
JournalNucleic Acids Research
Volume42
Issue number9
DOIs
StatePublished - 2014

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Adenosine Monophosphate
Histones
Protein Kinases
Chromatin
Genes
Hexosamines
Acetylglucosamine
Post Translational Protein Processing
Epigenomics
Phosphorylation
O-GlcNAc transferase
Food

ASJC Scopus subject areas

  • Genetics

Cite this

Xu, Q., Yang, C., Du, Y., Chen, Y., Liu, H., Deng, M., ... Pei, H. (2014). AMPK regulates histone H2B O-GlcNAcylation. Nucleic Acids Research, 42(9), 5594-5604. https://doi.org/10.1093/nar/gku236

AMPK regulates histone H2B O-GlcNAcylation. / Xu, Qiuran; Yang, Caihong; Du, Yu; Chen, Yali; Liu, Hailong; Deng, Min; Zhang, Haoxing; Zhang, Lei; Liu, Tongzheng; Liu, Qingguang; Wang, Liewei M; Lou, Zhenkun; Pei, Huadong.

In: Nucleic Acids Research, Vol. 42, No. 9, 2014, p. 5594-5604.

Research output: Contribution to journalArticle

Xu, Q, Yang, C, Du, Y, Chen, Y, Liu, H, Deng, M, Zhang, H, Zhang, L, Liu, T, Liu, Q, Wang, LM, Lou, Z & Pei, H 2014, 'AMPK regulates histone H2B O-GlcNAcylation', Nucleic Acids Research, vol. 42, no. 9, pp. 5594-5604. https://doi.org/10.1093/nar/gku236
Xu Q, Yang C, Du Y, Chen Y, Liu H, Deng M et al. AMPK regulates histone H2B O-GlcNAcylation. Nucleic Acids Research. 2014;42(9):5594-5604. https://doi.org/10.1093/nar/gku236
Xu, Qiuran ; Yang, Caihong ; Du, Yu ; Chen, Yali ; Liu, Hailong ; Deng, Min ; Zhang, Haoxing ; Zhang, Lei ; Liu, Tongzheng ; Liu, Qingguang ; Wang, Liewei M ; Lou, Zhenkun ; Pei, Huadong. / AMPK regulates histone H2B O-GlcNAcylation. In: Nucleic Acids Research. 2014 ; Vol. 42, No. 9. pp. 5594-5604.
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AU - Zhang, Haoxing

AU - Zhang, Lei

AU - Liu, Tongzheng

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