Amniotic Fluid Constituents, Cell Culture, and Neural Tube Defects

Daniel L. Van Dyke, Aubrey Milunsky

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Analysis of the chemical constituents of amniotic fluid (AF) has yielded valuable information for prenatal diagnosis, allowing assessment of fetal physiology and metabolism. Because the AF can be viewed as an extension of the fetal extracellular space, an understanding of its origin, formation, and chemical constitution is crucial to prenatal diagnosis and fetal therapy. This chapter lists various nonenzymatic constituents and characteristics of AF. Elevated values of biochemical constituents in AF may be nonspecific, such as 5-hydroxyindole-3-acetic acid in a fetus with Cornelia de Lange syndrome or acid-soluble glycoproteins in AF in CF. Reduced amounts of 5-hydroxyindole-3-acetic acid, and increased amino acids (especially methionine, isoleucine, leucine, tyrosine, and phenylalanine) have been observed in the AF of fetuses with neural tube defects (NTDs). The chapter also provides a synopsis of the current classification of human amniotic fluid cells (AFCs) in culture.

Original languageEnglish (US)
Title of host publicationGenetic Disorders and the Fetus: Diagnosis, Prevention and Treatment: Seventh Edition
Publisherwiley
Pages98-177
Number of pages80
ISBN (Electronic)9781118981559
ISBN (Print)9781118981528
DOIs
StatePublished - Jan 1 2016

Fingerprint

Neural Tube Defects
Amniotic Fluid
Cell Culture Techniques
Prenatal Diagnosis
Acetic Acid
Fetus
De Lange Syndrome
Fetal Therapies
Isoleucine
Constitution and Bylaws
Extracellular Space
Phenylalanine
Leucine
Methionine
Tyrosine
Glycoproteins
Amino Acids
Acids

Keywords

  • Amniotic fluid
  • Amniotic fluid α-fetoprotein
  • Cell culture
  • Neural tube defects
  • Prenatal diagnosis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Van Dyke, D. L., & Milunsky, A. (2016). Amniotic Fluid Constituents, Cell Culture, and Neural Tube Defects. In Genetic Disorders and the Fetus: Diagnosis, Prevention and Treatment: Seventh Edition (pp. 98-177). wiley. https://doi.org/10.1002/9781118981559.ch3

Amniotic Fluid Constituents, Cell Culture, and Neural Tube Defects. / Van Dyke, Daniel L.; Milunsky, Aubrey.

Genetic Disorders and the Fetus: Diagnosis, Prevention and Treatment: Seventh Edition. wiley, 2016. p. 98-177.

Research output: Chapter in Book/Report/Conference proceedingChapter

Van Dyke, DL & Milunsky, A 2016, Amniotic Fluid Constituents, Cell Culture, and Neural Tube Defects. in Genetic Disorders and the Fetus: Diagnosis, Prevention and Treatment: Seventh Edition. wiley, pp. 98-177. https://doi.org/10.1002/9781118981559.ch3
Van Dyke DL, Milunsky A. Amniotic Fluid Constituents, Cell Culture, and Neural Tube Defects. In Genetic Disorders and the Fetus: Diagnosis, Prevention and Treatment: Seventh Edition. wiley. 2016. p. 98-177 https://doi.org/10.1002/9781118981559.ch3
Van Dyke, Daniel L. ; Milunsky, Aubrey. / Amniotic Fluid Constituents, Cell Culture, and Neural Tube Defects. Genetic Disorders and the Fetus: Diagnosis, Prevention and Treatment: Seventh Edition. wiley, 2016. pp. 98-177
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