Amiodarone induces apoptosis in L-132 human lung epithelial cell line

In Serk Choi, Bum Shik Kim, Kyu Seok Cho, Joo Chul Park, Mi-Hyeon D Jang, Min Chul Shin, Sae Bin Jung, Joo Ho Chung, Chang Ju Kim

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

To investigate whether amiodarone induces apoptosis in cells of the L-132 human lung epithelial cell line, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, 4,6-diamidino-2-phenylindole staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction, and casapse-3 enzyme assay were performed. Through morphological and biochemical analyses, it was demonstrated that L-132 cells treated with amiodarone exhibit several features of apoptosis. In addition, it was shown that amiodarone increases the mRNA levels of bax and caspase-3. Based on the results, amiodarone appears to activate specific intracellular death-related pathways, including possibly the bax-dependent caspase-3 activation pathway, and thus induce apoptosis in human lung epithelial cells.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalToxicology Letters
Volume132
Issue number1
DOIs
StatePublished - Jun 7 2002
Externally publishedYes

    Fingerprint

Keywords

  • Amiodarone
  • Apoptosis
  • Bax
  • Caspase-3
  • Human lung epithelial cells

ASJC Scopus subject areas

  • Toxicology

Cite this

Choi, I. S., Kim, B. S., Cho, K. S., Park, J. C., Jang, M-H. D., Shin, M. C., Jung, S. B., Chung, J. H., & Kim, C. J. (2002). Amiodarone induces apoptosis in L-132 human lung epithelial cell line. Toxicology Letters, 132(1), 47-55. https://doi.org/10.1016/S0378-4274(02)00065-6