Abstract
To investigate whether amiodarone induces apoptosis in cells of the L-132 human lung epithelial cell line, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, 4,6-diamidino-2-phenylindole staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction, and casapse-3 enzyme assay were performed. Through morphological and biochemical analyses, it was demonstrated that L-132 cells treated with amiodarone exhibit several features of apoptosis. In addition, it was shown that amiodarone increases the mRNA levels of bax and caspase-3. Based on the results, amiodarone appears to activate specific intracellular death-related pathways, including possibly the bax-dependent caspase-3 activation pathway, and thus induce apoptosis in human lung epithelial cells.
Original language | English (US) |
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Pages (from-to) | 47-55 |
Number of pages | 9 |
Journal | Toxicology Letters |
Volume | 132 |
Issue number | 1 |
DOIs | |
State | Published - Jun 7 2002 |
Keywords
- Amiodarone
- Apoptosis
- Bax
- Caspase-3
- Human lung epithelial cells
ASJC Scopus subject areas
- Toxicology