Amiodarone induces apoptosis in L-132 human lung epithelial cell line

In Serk Choi, Bum Shik Kim, Kyu Seok Cho, Joo Chul Park, Mi Hyeon Jang, Min Chul Shin, Sae Bin Jung, Joo Ho Chung, Chang Ju Kim

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

To investigate whether amiodarone induces apoptosis in cells of the L-132 human lung epithelial cell line, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, 4,6-diamidino-2-phenylindole staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction, and casapse-3 enzyme assay were performed. Through morphological and biochemical analyses, it was demonstrated that L-132 cells treated with amiodarone exhibit several features of apoptosis. In addition, it was shown that amiodarone increases the mRNA levels of bax and caspase-3. Based on the results, amiodarone appears to activate specific intracellular death-related pathways, including possibly the bax-dependent caspase-3 activation pathway, and thus induce apoptosis in human lung epithelial cells.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalToxicology Letters
Volume132
Issue number1
DOIs
StatePublished - Jun 7 2002

Keywords

  • Amiodarone
  • Apoptosis
  • Bax
  • Caspase-3
  • Human lung epithelial cells

ASJC Scopus subject areas

  • Toxicology

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