Amiodarone induces apoptosis in L-132 human lung epithelial cell line

In Serk Choi, Bum Shik Kim, Kyu Seok Cho, Joo Chul Park, Mi-Hyeon D Jang, Min Chul Shin, Sae Bin Jung, Joo Ho Chung, Chang Ju Kim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To investigate whether amiodarone induces apoptosis in cells of the L-132 human lung epithelial cell line, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, 4,6-diamidino-2-phenylindole staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction, and casapse-3 enzyme assay were performed. Through morphological and biochemical analyses, it was demonstrated that L-132 cells treated with amiodarone exhibit several features of apoptosis. In addition, it was shown that amiodarone increases the mRNA levels of bax and caspase-3. Based on the results, amiodarone appears to activate specific intracellular death-related pathways, including possibly the bax-dependent caspase-3 activation pathway, and thus induce apoptosis in human lung epithelial cells.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalToxicology Letters
Volume132
Issue number1
DOIs
StatePublished - Jun 7 2002
Externally publishedYes

Fingerprint

Amiodarone
Assays
Epithelial Cells
Apoptosis
Cell Line
Lung
Caspase 3
DNA Nucleotidylexotransferase
Polymerase chain reaction
Enzyme Assays
DNA Fragmentation
Transcription
Labeling
Reverse Transcription
Chemical activation
Staining and Labeling
Polymerase Chain Reaction
Messenger RNA
DNA
Enzymes

Keywords

  • Amiodarone
  • Apoptosis
  • Bax
  • Caspase-3
  • Human lung epithelial cells

ASJC Scopus subject areas

  • Toxicology

Cite this

Choi, I. S., Kim, B. S., Cho, K. S., Park, J. C., Jang, M-H. D., Shin, M. C., ... Kim, C. J. (2002). Amiodarone induces apoptosis in L-132 human lung epithelial cell line. Toxicology Letters, 132(1), 47-55. https://doi.org/10.1016/S0378-4274(02)00065-6

Amiodarone induces apoptosis in L-132 human lung epithelial cell line. / Choi, In Serk; Kim, Bum Shik; Cho, Kyu Seok; Park, Joo Chul; Jang, Mi-Hyeon D; Shin, Min Chul; Jung, Sae Bin; Chung, Joo Ho; Kim, Chang Ju.

In: Toxicology Letters, Vol. 132, No. 1, 07.06.2002, p. 47-55.

Research output: Contribution to journalArticle

Choi, IS, Kim, BS, Cho, KS, Park, JC, Jang, M-HD, Shin, MC, Jung, SB, Chung, JH & Kim, CJ 2002, 'Amiodarone induces apoptosis in L-132 human lung epithelial cell line', Toxicology Letters, vol. 132, no. 1, pp. 47-55. https://doi.org/10.1016/S0378-4274(02)00065-6
Choi, In Serk ; Kim, Bum Shik ; Cho, Kyu Seok ; Park, Joo Chul ; Jang, Mi-Hyeon D ; Shin, Min Chul ; Jung, Sae Bin ; Chung, Joo Ho ; Kim, Chang Ju. / Amiodarone induces apoptosis in L-132 human lung epithelial cell line. In: Toxicology Letters. 2002 ; Vol. 132, No. 1. pp. 47-55.
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