Amino acid substitution at peptide-binding pockets of HLA class I molecules increases risk of severe acute GVHD and mortality

Joseph Pidala, Tao Wang, Michael Haagenson, Stephen R. Spellman, Medhat Askar, Minoo Battiwalla, Lee Ann Baxter-Lowe, Menachem Bitan, Marcelo Fernandez-Viña, Manish Gandhi, Ann A. Jakubowski, Martin Maiers, Susana R. Marino, Steven G.E. Marsh, Machteld Oudshoorn, Jeanne Palmer, Vinod K. Prasad, Vijay Reddy, Olle Ringden, Wael SaberStella Santarone, Kirk R. Schultz, Michelle Setterholm, Elizabeth Trachtenberg, E. Victoria Turner, Ann E. Woolfrey, Stephanie J. Lee, Claudio Anasetti

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

HLA disparity has a negative impact on the outcomes of hematopoietic cell transplantation (HCT). We studied the independent impact of amino acid substitution (AAS) at peptidebinding positions 9, 99, 116, and 156, and killer immunoglobulin-like receptor binding position 77 of HLA-A, B, or C, on the risks for grade 3-4 acute graft-versus-host disease (GVHD), chronic GVHD, treatment-related mortality (TRM), relapse, and overall survival. In multivariate analysis, a mismatch at HLA-C position 116 was associated with increased risk for severe acute GVHD (hazard ratio [HR] = 1.45, 95% confidence interval [CI] = 1.15-1.82, P =.0016). Mismatch at HLA-C position 99 was associated with increased transplantrelated mortality (HR = 1.37, 95% CI = 1.1-1.69, P =.0038). Mismatch at HLA-B position 9 was associated with increased chronic GVHD (HR=2.28, 95%CI =1.36-3.82, P=.0018).No AAS were significantly associated with outcome at HLA-A. Specific AAS pair combinations with a frequency >30 were tested for association with HCT outcomes. Cysteine to tyrosine substitution at position 99 of HLA-C was associated with increased TRM (HR = 1.78, 95% = CI 1.27-2.51, P =.0009). These results demonstrate that donor-recipient mismatch for certainpeptide-binding residues of the HLA class Imoleculeis associated with increased risk for acute and chronic GVHD and death.

Original languageEnglish (US)
Pages (from-to)3651-3658
Number of pages8
JournalBlood
Volume122
Issue number22
DOIs
StatePublished - Nov 21 2013
Externally publishedYes

Fingerprint

HLA-C Antigens
Graft vs Host Disease
Amino Acid Substitution
Grafts
Substitution reactions
Hazards
Amino Acids
Peptides
Molecules
Mortality
Confidence Intervals
HLA-A Antigens
HLA-B Antigens
Cell Transplantation
KIR Receptors
Cysteine
Tyrosine
Multivariate Analysis
Association reactions
Recurrence

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Pidala, J., Wang, T., Haagenson, M., Spellman, S. R., Askar, M., Battiwalla, M., ... Anasetti, C. (2013). Amino acid substitution at peptide-binding pockets of HLA class I molecules increases risk of severe acute GVHD and mortality. Blood, 122(22), 3651-3658. https://doi.org/10.1182/blood-2013-05-501510

Amino acid substitution at peptide-binding pockets of HLA class I molecules increases risk of severe acute GVHD and mortality. / Pidala, Joseph; Wang, Tao; Haagenson, Michael; Spellman, Stephen R.; Askar, Medhat; Battiwalla, Minoo; Baxter-Lowe, Lee Ann; Bitan, Menachem; Fernandez-Viña, Marcelo; Gandhi, Manish; Jakubowski, Ann A.; Maiers, Martin; Marino, Susana R.; Marsh, Steven G.E.; Oudshoorn, Machteld; Palmer, Jeanne; Prasad, Vinod K.; Reddy, Vijay; Ringden, Olle; Saber, Wael; Santarone, Stella; Schultz, Kirk R.; Setterholm, Michelle; Trachtenberg, Elizabeth; Turner, E. Victoria; Woolfrey, Ann E.; Lee, Stephanie J.; Anasetti, Claudio.

In: Blood, Vol. 122, No. 22, 21.11.2013, p. 3651-3658.

Research output: Contribution to journalArticle

Pidala, J, Wang, T, Haagenson, M, Spellman, SR, Askar, M, Battiwalla, M, Baxter-Lowe, LA, Bitan, M, Fernandez-Viña, M, Gandhi, M, Jakubowski, AA, Maiers, M, Marino, SR, Marsh, SGE, Oudshoorn, M, Palmer, J, Prasad, VK, Reddy, V, Ringden, O, Saber, W, Santarone, S, Schultz, KR, Setterholm, M, Trachtenberg, E, Turner, EV, Woolfrey, AE, Lee, SJ & Anasetti, C 2013, 'Amino acid substitution at peptide-binding pockets of HLA class I molecules increases risk of severe acute GVHD and mortality', Blood, vol. 122, no. 22, pp. 3651-3658. https://doi.org/10.1182/blood-2013-05-501510
Pidala, Joseph ; Wang, Tao ; Haagenson, Michael ; Spellman, Stephen R. ; Askar, Medhat ; Battiwalla, Minoo ; Baxter-Lowe, Lee Ann ; Bitan, Menachem ; Fernandez-Viña, Marcelo ; Gandhi, Manish ; Jakubowski, Ann A. ; Maiers, Martin ; Marino, Susana R. ; Marsh, Steven G.E. ; Oudshoorn, Machteld ; Palmer, Jeanne ; Prasad, Vinod K. ; Reddy, Vijay ; Ringden, Olle ; Saber, Wael ; Santarone, Stella ; Schultz, Kirk R. ; Setterholm, Michelle ; Trachtenberg, Elizabeth ; Turner, E. Victoria ; Woolfrey, Ann E. ; Lee, Stephanie J. ; Anasetti, Claudio. / Amino acid substitution at peptide-binding pockets of HLA class I molecules increases risk of severe acute GVHD and mortality. In: Blood. 2013 ; Vol. 122, No. 22. pp. 3651-3658.
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abstract = "HLA disparity has a negative impact on the outcomes of hematopoietic cell transplantation (HCT). We studied the independent impact of amino acid substitution (AAS) at peptidebinding positions 9, 99, 116, and 156, and killer immunoglobulin-like receptor binding position 77 of HLA-A, B, or C, on the risks for grade 3-4 acute graft-versus-host disease (GVHD), chronic GVHD, treatment-related mortality (TRM), relapse, and overall survival. In multivariate analysis, a mismatch at HLA-C position 116 was associated with increased risk for severe acute GVHD (hazard ratio [HR] = 1.45, 95{\%} confidence interval [CI] = 1.15-1.82, P =.0016). Mismatch at HLA-C position 99 was associated with increased transplantrelated mortality (HR = 1.37, 95{\%} CI = 1.1-1.69, P =.0038). Mismatch at HLA-B position 9 was associated with increased chronic GVHD (HR=2.28, 95{\%}CI =1.36-3.82, P=.0018).No AAS were significantly associated with outcome at HLA-A. Specific AAS pair combinations with a frequency >30 were tested for association with HCT outcomes. Cysteine to tyrosine substitution at position 99 of HLA-C was associated with increased TRM (HR = 1.78, 95{\%} = CI 1.27-2.51, P =.0009). These results demonstrate that donor-recipient mismatch for certainpeptide-binding residues of the HLA class Imoleculeis associated with increased risk for acute and chronic GVHD and death.",
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T1 - Amino acid substitution at peptide-binding pockets of HLA class I molecules increases risk of severe acute GVHD and mortality

AU - Pidala, Joseph

AU - Wang, Tao

AU - Haagenson, Michael

AU - Spellman, Stephen R.

AU - Askar, Medhat

AU - Battiwalla, Minoo

AU - Baxter-Lowe, Lee Ann

AU - Bitan, Menachem

AU - Fernandez-Viña, Marcelo

AU - Gandhi, Manish

AU - Jakubowski, Ann A.

AU - Maiers, Martin

AU - Marino, Susana R.

AU - Marsh, Steven G.E.

AU - Oudshoorn, Machteld

AU - Palmer, Jeanne

AU - Prasad, Vinod K.

AU - Reddy, Vijay

AU - Ringden, Olle

AU - Saber, Wael

AU - Santarone, Stella

AU - Schultz, Kirk R.

AU - Setterholm, Michelle

AU - Trachtenberg, Elizabeth

AU - Turner, E. Victoria

AU - Woolfrey, Ann E.

AU - Lee, Stephanie J.

AU - Anasetti, Claudio

PY - 2013/11/21

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N2 - HLA disparity has a negative impact on the outcomes of hematopoietic cell transplantation (HCT). We studied the independent impact of amino acid substitution (AAS) at peptidebinding positions 9, 99, 116, and 156, and killer immunoglobulin-like receptor binding position 77 of HLA-A, B, or C, on the risks for grade 3-4 acute graft-versus-host disease (GVHD), chronic GVHD, treatment-related mortality (TRM), relapse, and overall survival. In multivariate analysis, a mismatch at HLA-C position 116 was associated with increased risk for severe acute GVHD (hazard ratio [HR] = 1.45, 95% confidence interval [CI] = 1.15-1.82, P =.0016). Mismatch at HLA-C position 99 was associated with increased transplantrelated mortality (HR = 1.37, 95% CI = 1.1-1.69, P =.0038). Mismatch at HLA-B position 9 was associated with increased chronic GVHD (HR=2.28, 95%CI =1.36-3.82, P=.0018).No AAS were significantly associated with outcome at HLA-A. Specific AAS pair combinations with a frequency >30 were tested for association with HCT outcomes. Cysteine to tyrosine substitution at position 99 of HLA-C was associated with increased TRM (HR = 1.78, 95% = CI 1.27-2.51, P =.0009). These results demonstrate that donor-recipient mismatch for certainpeptide-binding residues of the HLA class Imoleculeis associated with increased risk for acute and chronic GVHD and death.

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