Amino-acid levels in subretinal and vitreous fluid of patients with retinal detachment

K. M. Bertram; D. V. Bula; J. S. Pulido; S. A. Shippy; S. Gautam; M. J. Lu; R. M. Hatfield; J. H. Kim; M. T. Quirk; J. G. Arroyo

doi:10.1038/sj.eye.6702993

Amino-acid levels in subretinal and vitreous fluid of patients with retinal detachment

K. M. Bertram, D. V. Bula, J. S. Pulido, S. A. Shippy, S. Gautam, M. J. Lu, R. M. Hatfield, J. H. Kim, M. T. Quirk, J. G. Arroyo

Ophthalmology

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Purpose: To compare the concentration of amino acids in subretinal and vitreous fluid of patients with primary rhegmatogenous retinal detachment to that of control vitreous. Methods: This prospective, observational study measured amino-acid levels in subretinal fluid of patients undergoing scleral buckle placement (n = 20) and vitreous fluid in patients undergoing pars plana vitrectomy (n = 5) for primary retinal detachment. Vitreous fluid from patients undergoing vitrectomy for macular hole (n = 7) or epiretinal membrane (n = 3) served as a control. Subretinal fluid and control vitreous were analysed using high-pressure liquid chromatography. Retinal detachment vitreous was analysed using capillary electrophoresis-laser-induced fluorescence. Results: Mean levels of glutamate (27.0 ± 1.7 μM), aspartate (4.1 ± 4.0 μM), and glycine (44.1 ± 31.0 μM) in subretinal fluid and glutamate (13.4 ± 11.9 μM) in the vitreous were significantly elevated in retinal detachment compared to control vitreous. A significant, positive association was observed between levels of aspartate and glutamate in subretinal fluid (Spearman's correlation coefficient: 0.74, P < 0.01). Mean arginine levels did not differ significantly between subretinal fluid and control vitreous. Levels of alanine, tyrosine, valine, isoleucine, leucine, and phenylalanine were significantly lower in subretinal fluid compared to control vitreous (all P < 0.01). Conclusions: Glutamate levels in subretinal fluid and vitreous of patients with primary retinal detachment is significantly elevated in comparison to control vitreous. This finding lends further support to the hypothesis that elevated glutamate levels may result from ischaemia of the outer retina secondary to retinal detachment.

Original language	English (US)
Pages (from-to)	582-589
Number of pages	8
Journal	Eye
Volume	22
Issue number	4
DOIs	https://doi.org/10.1038/sj.eye.6702993
State	Published - Apr 2008

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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@article{a8e004473272413893c4ed7c96dd6165,

title = "Amino-acid levels in subretinal and vitreous fluid of patients with retinal detachment",

abstract = "Purpose: To compare the concentration of amino acids in subretinal and vitreous fluid of patients with primary rhegmatogenous retinal detachment to that of control vitreous. Methods: This prospective, observational study measured amino-acid levels in subretinal fluid of patients undergoing scleral buckle placement (n = 20) and vitreous fluid in patients undergoing pars plana vitrectomy (n = 5) for primary retinal detachment. Vitreous fluid from patients undergoing vitrectomy for macular hole (n = 7) or epiretinal membrane (n = 3) served as a control. Subretinal fluid and control vitreous were analysed using high-pressure liquid chromatography. Retinal detachment vitreous was analysed using capillary electrophoresis-laser-induced fluorescence. Results: Mean levels of glutamate (27.0 ± 1.7 μM), aspartate (4.1 ± 4.0 μM), and glycine (44.1 ± 31.0 μM) in subretinal fluid and glutamate (13.4 ± 11.9 μM) in the vitreous were significantly elevated in retinal detachment compared to control vitreous. A significant, positive association was observed between levels of aspartate and glutamate in subretinal fluid (Spearman's correlation coefficient: 0.74, P < 0.01). Mean arginine levels did not differ significantly between subretinal fluid and control vitreous. Levels of alanine, tyrosine, valine, isoleucine, leucine, and phenylalanine were significantly lower in subretinal fluid compared to control vitreous (all P < 0.01). Conclusions: Glutamate levels in subretinal fluid and vitreous of patients with primary retinal detachment is significantly elevated in comparison to control vitreous. This finding lends further support to the hypothesis that elevated glutamate levels may result from ischaemia of the outer retina secondary to retinal detachment.",

author = "Bertram, {K. M.} and Bula, {D. V.} and Pulido, {J. S.} and Shippy, {S. A.} and S. Gautam and Lu, {M. J.} and Hatfield, {R. M.} and Kim, {J. H.} and Quirk, {M. T.} and Arroyo, {J. G.}",

note = "Funding Information: Amino-acid analyses of SRF and control vitreous fluid were done at the WM Keck Foundation Biotechnology Resource Laboratory of Yale University (New Haven, CT, USA). Retinal detachment vitreous fluid was analysed in the Department of Chemistry, University of Illinois at Chicago (Chicago, IL, USA). M-JL, JSP, and SAS gratefully acknowledge financial support from NIH EY014908. The authors declare that they have no proprietary interest in this research. Dr Arroyo is the recipient of an NIH K-23 Physician Training Award. This work was also supported by the Grmstraw-Code WSCZ Charitable Foundation.",

year = "2008",

month = apr,

doi = "10.1038/sj.eye.6702993",

language = "English (US)",

volume = "22",

pages = "582--589",

journal = "Eye",

issn = "0950-222X",

publisher = "Nature Publishing Group",

number = "4",

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T1 - Amino-acid levels in subretinal and vitreous fluid of patients with retinal detachment

AU - Bertram, K. M.

AU - Bula, D. V.

AU - Pulido, J. S.

AU - Shippy, S. A.

AU - Gautam, S.

AU - Lu, M. J.

AU - Hatfield, R. M.

AU - Kim, J. H.

AU - Quirk, M. T.

AU - Arroyo, J. G.

N1 - Funding Information: Amino-acid analyses of SRF and control vitreous fluid were done at the WM Keck Foundation Biotechnology Resource Laboratory of Yale University (New Haven, CT, USA). Retinal detachment vitreous fluid was analysed in the Department of Chemistry, University of Illinois at Chicago (Chicago, IL, USA). M-JL, JSP, and SAS gratefully acknowledge financial support from NIH EY014908. The authors declare that they have no proprietary interest in this research. Dr Arroyo is the recipient of an NIH K-23 Physician Training Award. This work was also supported by the Grmstraw-Code WSCZ Charitable Foundation.

PY - 2008/4

Y1 - 2008/4

N2 - Purpose: To compare the concentration of amino acids in subretinal and vitreous fluid of patients with primary rhegmatogenous retinal detachment to that of control vitreous. Methods: This prospective, observational study measured amino-acid levels in subretinal fluid of patients undergoing scleral buckle placement (n = 20) and vitreous fluid in patients undergoing pars plana vitrectomy (n = 5) for primary retinal detachment. Vitreous fluid from patients undergoing vitrectomy for macular hole (n = 7) or epiretinal membrane (n = 3) served as a control. Subretinal fluid and control vitreous were analysed using high-pressure liquid chromatography. Retinal detachment vitreous was analysed using capillary electrophoresis-laser-induced fluorescence. Results: Mean levels of glutamate (27.0 ± 1.7 μM), aspartate (4.1 ± 4.0 μM), and glycine (44.1 ± 31.0 μM) in subretinal fluid and glutamate (13.4 ± 11.9 μM) in the vitreous were significantly elevated in retinal detachment compared to control vitreous. A significant, positive association was observed between levels of aspartate and glutamate in subretinal fluid (Spearman's correlation coefficient: 0.74, P < 0.01). Mean arginine levels did not differ significantly between subretinal fluid and control vitreous. Levels of alanine, tyrosine, valine, isoleucine, leucine, and phenylalanine were significantly lower in subretinal fluid compared to control vitreous (all P < 0.01). Conclusions: Glutamate levels in subretinal fluid and vitreous of patients with primary retinal detachment is significantly elevated in comparison to control vitreous. This finding lends further support to the hypothesis that elevated glutamate levels may result from ischaemia of the outer retina secondary to retinal detachment.

AB - Purpose: To compare the concentration of amino acids in subretinal and vitreous fluid of patients with primary rhegmatogenous retinal detachment to that of control vitreous. Methods: This prospective, observational study measured amino-acid levels in subretinal fluid of patients undergoing scleral buckle placement (n = 20) and vitreous fluid in patients undergoing pars plana vitrectomy (n = 5) for primary retinal detachment. Vitreous fluid from patients undergoing vitrectomy for macular hole (n = 7) or epiretinal membrane (n = 3) served as a control. Subretinal fluid and control vitreous were analysed using high-pressure liquid chromatography. Retinal detachment vitreous was analysed using capillary electrophoresis-laser-induced fluorescence. Results: Mean levels of glutamate (27.0 ± 1.7 μM), aspartate (4.1 ± 4.0 μM), and glycine (44.1 ± 31.0 μM) in subretinal fluid and glutamate (13.4 ± 11.9 μM) in the vitreous were significantly elevated in retinal detachment compared to control vitreous. A significant, positive association was observed between levels of aspartate and glutamate in subretinal fluid (Spearman's correlation coefficient: 0.74, P < 0.01). Mean arginine levels did not differ significantly between subretinal fluid and control vitreous. Levels of alanine, tyrosine, valine, isoleucine, leucine, and phenylalanine were significantly lower in subretinal fluid compared to control vitreous (all P < 0.01). Conclusions: Glutamate levels in subretinal fluid and vitreous of patients with primary retinal detachment is significantly elevated in comparison to control vitreous. This finding lends further support to the hypothesis that elevated glutamate levels may result from ischaemia of the outer retina secondary to retinal detachment.

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Amino-acid levels in subretinal and vitreous fluid of patients with retinal detachment

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