American ginseng (Panax quinquefolius) administration does not affect performance of the Roche COBAS Ampliprep/Taqman HIV-1 RNA assay

Rahul P. Bakshi, Todd T. Brown, Antoine Simmons, Chun Su Yuan, Brent A. Bauer, Jeff A. Sloan, Adriana Andrade

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Background: Previous data indicate that purified components of ginseng can inhibit HIV reverse transcriptase in vitro, suggesting that ginseng components in plasma may interfere with HIV-1 RNA detection assays. Methods: Pre- and post-dose plasma from three volunteers dosed with 3000 mg American ginseng was spiked with HIV and analyzed by the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. Results: Presence of American ginseng had no significant effect on measured HIV-1 RNA concentration. Variation within pre- and post-dose plasma pair was insignificant and within assay performance limits. Conclusion: Plasma from subjects dosed with 3000 mg American ginseng does not interfere with the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. This implies that in vitro inhibition of HIV reverse transcriptase by American ginseng components is unlikely to be clinically relevant.

Original languageEnglish (US)
Article number427
JournalBMC Complementary and Alternative Medicine
Volume14
Issue number1
DOIs
StatePublished - Oct 31 2014

ASJC Scopus subject areas

  • Complementary and alternative medicine

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