American ginseng (Panax quinquefolius) administration does not affect performance of the Roche COBAS Ampliprep/Taqman HIV-1 RNA assay

Rahul P. Bakshi, Todd T. Brown, Antoine Simmons, Chun Su Yuan, Brent A Bauer, Jeff A Sloan, Adriana Andrade

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Previous data indicate that purified components of ginseng can inhibit HIV reverse transcriptase in vitro, suggesting that ginseng components in plasma may interfere with HIV-1 RNA detection assays. Methods: Pre- and post-dose plasma from three volunteers dosed with 3000 mg American ginseng was spiked with HIV and analyzed by the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. Results: Presence of American ginseng had no significant effect on measured HIV-1 RNA concentration. Variation within pre- and post-dose plasma pair was insignificant and within assay performance limits. Conclusion: Plasma from subjects dosed with 3000 mg American ginseng does not interfere with the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. This implies that in vitro inhibition of HIV reverse transcriptase by American ginseng components is unlikely to be clinically relevant.

Original languageEnglish (US)
Pages (from-to)427
Number of pages1
JournalBMC Complementary and Alternative Medicine
DOIs
StateAccepted/In press - Oct 31 2014

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Panax
HIV-1
RNA
HIV Reverse Transcriptase
Volunteers
HIV

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Medicine(all)

Cite this

American ginseng (Panax quinquefolius) administration does not affect performance of the Roche COBAS Ampliprep/Taqman HIV-1 RNA assay. / Bakshi, Rahul P.; Brown, Todd T.; Simmons, Antoine; Yuan, Chun Su; Bauer, Brent A; Sloan, Jeff A; Andrade, Adriana.

In: BMC Complementary and Alternative Medicine, 31.10.2014, p. 427.

Research output: Contribution to journalArticle

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abstract = "Background: Previous data indicate that purified components of ginseng can inhibit HIV reverse transcriptase in vitro, suggesting that ginseng components in plasma may interfere with HIV-1 RNA detection assays. Methods: Pre- and post-dose plasma from three volunteers dosed with 3000 mg American ginseng was spiked with HIV and analyzed by the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. Results: Presence of American ginseng had no significant effect on measured HIV-1 RNA concentration. Variation within pre- and post-dose plasma pair was insignificant and within assay performance limits. Conclusion: Plasma from subjects dosed with 3000 mg American ginseng does not interfere with the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. This implies that in vitro inhibition of HIV reverse transcriptase by American ginseng components is unlikely to be clinically relevant.",
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AU - Andrade, Adriana

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