Alz 50, a monoclonal antibody to Alzheimer's disease antigen, cross-reacts with τ proteins from bovine and normal human brain

H. Ksiezak-Reding, P. Davies, S. H. Yen

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Abstract

Microtubule-associated protein τ from bovine brain reacted on immunoblots and on enzyme-linked immunosorbent assay with a monoclonal antibody, Alz 50, which has previously been found to bind to an Alzheimer disease-specific antigen. The apparent affinity of binding of Alz 50 to τ was 2.1 x 10 -9 M on competitive enzyme-linked immunosorbent assay, and it was in the same range as for Tau-1 (0.5 x 10 -9 M), an antibody raised against purified bovine τ proteins. Immunoblotting of trypsin-digested τ revealed differences between Alz 50 and Tau-1 binding sites. The binding of both antibodies to τ was not affected by prior treatment with phosphatase, indicating that the cross-reactivity of Alz 50 with τ is due to the presence of phosphate-independent epitope. This epitope then differs from phosphate-dependent τ epitopes often shared with other cytoskeletal proteins. Alz 50 and Tau-1 binding sites were present in all isoelectric (pI 6-8) and molecular weight variants of τ. In contrast, phosphate-dependent epitopes recognized by another τ-reactive antibody (NP 14) were found mostly in acidic τ variants. Similarly to τ proteins from bovine brain, τ-enriched preparations from normal human brain contained Alz 50 and Tau-1 reactive sites in all isoelectric (pI 6.5-8.5) and molecular weight variants. Our observation of Alz 50 cross-reactivity with τ suggests a relationship between τ and the novel protein identified recently in Alzheimer brains.

Original languageEnglish (US)
Pages (from-to)7943-7947
Number of pages5
JournalJournal of Biological Chemistry
Volume263
Issue number17
StatePublished - 1988
Externally publishedYes

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Epitopes
Brain
Monoclonal Antibodies
Immunosorbents
Phosphates
Antibodies
Assays
Proteins
Molecular Weight
Enzyme-Linked Immunosorbent Assay
Molecular weight
Binding Sites
Microtubule-Associated Proteins
Cytoskeletal Proteins
Enzymes
Phosphoric Monoester Hydrolases
Immunoblotting
Trypsin
Catalytic Domain
Observation

ASJC Scopus subject areas

  • Biochemistry

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Alz 50, a monoclonal antibody to Alzheimer's disease antigen, cross-reacts with τ proteins from bovine and normal human brain. / Ksiezak-Reding, H.; Davies, P.; Yen, S. H.

In: Journal of Biological Chemistry, Vol. 263, No. 17, 1988, p. 7943-7947.

Research output: Contribution to journalArticle

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AU - Davies, P.

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