Alternative pathways for the sorting of soluble vacuolar proteins in yeast: A vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases

G. Paravicini, Bruce F Horazdovsky, S. D. Emr

Research output: Contribution to journalArticle

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Abstract

vps35 mutants of Saccharomyces cerevisiae exhibit severe defects in the localization of carboxypeptidase Y, a soluble vacuolar hydrolase. We have cloned the wild-type VPS35 gene by complementation of the vacuolar protein sorting defect exhibited by the vps35-17 mutant. Sequence analysis revealed an open reading frame predicted to encode a protein of 937 amino acids that lacks any obvious hydrophobic domains. Subcellular fractionation studies indicated that 80% of Vps35p peripherally associates with a membranous particulate cell fraction. The association of Vps35p with this fraction appears to be saturable; when overproduced, the vast majority of Vps35p remains in a soluble fraction. Disruption of the VPS35 gene demonstrated that it is not essential for yeast cell growth. However, the null allele of VPS35 results in a differential defect in the sorting of vacuolar carboxypeptidase Y (CPY), proteinase A (PrA), proteinase B (PrB), and alkaline phosphatase (ALP). proCPY was quantitatively missorted and secreted by Δvps35 cells, whereas almost all of proPrA, proPrB, and proALP were retained within the cell and converted to their mature forms, indicating delivery to the vacuole. Based on these observations, we propose that alternative pathways exist for the sorting and/or delivery of proteins to the vacuole.

Original languageEnglish (US)
Pages (from-to)415-427
Number of pages13
JournalMolecular Biology of the Cell
Volume3
Issue number4
StatePublished - 1992
Externally publishedYes

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Fungal Proteins
Hydrolases
Cathepsin A
yeast proteinase B
Vacuoles
Protein Transport
Open Reading Frames
Genes
Alkaline Phosphatase
Sequence Analysis
Saccharomyces cerevisiae
Proteins
Peptide Hydrolases
Yeasts
Alleles
Amino Acids
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Alternative pathways for the sorting of soluble vacuolar proteins in yeast : A vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases. / Paravicini, G.; Horazdovsky, Bruce F; Emr, S. D.

In: Molecular Biology of the Cell, Vol. 3, No. 4, 1992, p. 415-427.

Research output: Contribution to journalArticle

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