Alternative 3′ UTR polyadenylation of Bzw1 transcripts display differential translation efficiency and tissue-specific expression

Mingyan Yu; Haibo Sha; Yan Gao; Hu Zeng; Minsheng Zhu; Xiang Gao

doi:10.1016/j.bbrc.2006.04.113

Alternative 3′ UTR polyadenylation of Bzw1 transcripts display differential translation efficiency and tissue-specific expression

Mingyan Yu, Haibo Sha, Yan Gao, Hu Zeng, Minsheng Zhu, Xiang Gao

Rheumatology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

BZW1 is a conserved regulatory factor for transcriptional control of histone H4 gene at the G1/S transition. In this study, three Bzw1 transcripts were identified in mice with two long forms (∼2.9 kb) expressed ubiquitously at low level, and a short transcript of 1.8 kb expressed at high level exclusively in testis. These different transcripts share the same 5′ UTR and coding sequence, but differ in the length of 3′ UTR by utilizing alternative polyadenylation sites. Different translation efficiencies were observed in the cells transfected with chimeric EGFP-Bzw1 genes tailed with different 3′ UTRs. Our results demonstrate that Bzw1 transcripts are alternatively polyadenylated and expressed in tissue-specific pattern.

Original language	English (US)
Pages (from-to)	479-485
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	345
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.04.113
State	Published - Jun 23 2006

Keywords

3′ UTR
Alternative polyadenylation site
Bzw1
Testis
Translational efficiency

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.04.113

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title = "Alternative 3′ UTR polyadenylation of Bzw1 transcripts display differential translation efficiency and tissue-specific expression",

abstract = "BZW1 is a conserved regulatory factor for transcriptional control of histone H4 gene at the G1/S transition. In this study, three Bzw1 transcripts were identified in mice with two long forms (∼2.9 kb) expressed ubiquitously at low level, and a short transcript of 1.8 kb expressed at high level exclusively in testis. These different transcripts share the same 5′ UTR and coding sequence, but differ in the length of 3′ UTR by utilizing alternative polyadenylation sites. Different translation efficiencies were observed in the cells transfected with chimeric EGFP-Bzw1 genes tailed with different 3′ UTRs. Our results demonstrate that Bzw1 transcripts are alternatively polyadenylated and expressed in tissue-specific pattern.",

keywords = "3′ UTR, Alternative polyadenylation site, Bzw1, Testis, Translational efficiency",

author = "Mingyan Yu and Haibo Sha and Yan Gao and Hu Zeng and Minsheng Zhu and Xiang Gao",

note = "Funding Information: The authors thank Drs. Wei Xie, Qingshun Zhao, Zhongzhou Yang, and Xingxing Gu for their suggestions. This work is supported in part by E-Institutes of Shanghai Municipal Education Commission (E03003), National 973 project (2005CB522501) and NSFC grant (30370714) to Xiang Gao.",

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AU - Sha, Haibo

AU - Gao, Yan

AU - Zeng, Hu

AU - Zhu, Minsheng

AU - Gao, Xiang

N1 - Funding Information: The authors thank Drs. Wei Xie, Qingshun Zhao, Zhongzhou Yang, and Xingxing Gu for their suggestions. This work is supported in part by E-Institutes of Shanghai Municipal Education Commission (E03003), National 973 project (2005CB522501) and NSFC grant (30370714) to Xiang Gao.

PY - 2006/6/23

Y1 - 2006/6/23

N2 - BZW1 is a conserved regulatory factor for transcriptional control of histone H4 gene at the G1/S transition. In this study, three Bzw1 transcripts were identified in mice with two long forms (∼2.9 kb) expressed ubiquitously at low level, and a short transcript of 1.8 kb expressed at high level exclusively in testis. These different transcripts share the same 5′ UTR and coding sequence, but differ in the length of 3′ UTR by utilizing alternative polyadenylation sites. Different translation efficiencies were observed in the cells transfected with chimeric EGFP-Bzw1 genes tailed with different 3′ UTRs. Our results demonstrate that Bzw1 transcripts are alternatively polyadenylated and expressed in tissue-specific pattern.

AB - BZW1 is a conserved regulatory factor for transcriptional control of histone H4 gene at the G1/S transition. In this study, three Bzw1 transcripts were identified in mice with two long forms (∼2.9 kb) expressed ubiquitously at low level, and a short transcript of 1.8 kb expressed at high level exclusively in testis. These different transcripts share the same 5′ UTR and coding sequence, but differ in the length of 3′ UTR by utilizing alternative polyadenylation sites. Different translation efficiencies were observed in the cells transfected with chimeric EGFP-Bzw1 genes tailed with different 3′ UTRs. Our results demonstrate that Bzw1 transcripts are alternatively polyadenylated and expressed in tissue-specific pattern.

KW - 3′ UTR

KW - Alternative polyadenylation site

KW - Bzw1

KW - Testis

KW - Translational efficiency

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Alternative 3′ UTR polyadenylation of Bzw1 transcripts display differential translation efficiency and tissue-specific expression

Abstract

Keywords

ASJC Scopus subject areas

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