Altered transcription of a defective measles virus genome derived from a diseased human brain.

Roberto Cattaneo, G. Rebmann, A. Schmid, K. Baczko, V. ter Meulen, M. A. Billeter

Research output: Contribution to journalArticle

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Abstract

Measles virus (MV) is a negative strand RNA virus which usually causes acute disease, but in rare cases its persistence in the human brain induces the lethal disease subacute sclerosing panencephalitis (SSPE). The transcription of MV and of a defective MV derived from autopsy material of a SSPE case was studied in cultured cells. In the lytic infection the levels of the MV mRNAs decreased progressively with the distance of the six cognate genes from the 3' end of the genome, reflecting transcriptional attenuation at every gene junction. Transcripts covering two or three adjacent genes accounted for up to 20% of single gene transcripts; incidentally the MV intergenic transcription signals were found to be less conserved than the analogous signals of other negative strand RNA viruses. Although the analysed SSPE-derived defective MV showed a localized transcription defect at the phosphoprotein--matrix gene junction (substitution of the mRNAs by readthrough transcripts), the corresponding intergenic 'consensus' sequence and the surrounding nucleotides were not altered. This implies that factor(s) involved in the transcription of this defective SSPE virus fail to recognize this particular signal sequence, a constellation which in this and other cases might be causally related to the development of MV persistence.

Original languageEnglish (US)
Pages (from-to)681-688
Number of pages8
JournalEMBO Journal
Volume6
Issue number3
StatePublished - Mar 1987
Externally publishedYes

Fingerprint

Defective Viruses
Measles virus
Brain Diseases
Transcription
Viruses
Brain
Subacute Sclerosing Panencephalitis
Genes
Genome
RNA Viruses
Messenger RNA
Intergenic DNA
Phosphoproteins
Consensus Sequence
Acute Disease
Protein Sorting Signals
RNA
Autopsy
Cultured Cells
Nucleotides

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Cattaneo, R., Rebmann, G., Schmid, A., Baczko, K., ter Meulen, V., & Billeter, M. A. (1987). Altered transcription of a defective measles virus genome derived from a diseased human brain. EMBO Journal, 6(3), 681-688.

Altered transcription of a defective measles virus genome derived from a diseased human brain. / Cattaneo, Roberto; Rebmann, G.; Schmid, A.; Baczko, K.; ter Meulen, V.; Billeter, M. A.

In: EMBO Journal, Vol. 6, No. 3, 03.1987, p. 681-688.

Research output: Contribution to journalArticle

Cattaneo, R, Rebmann, G, Schmid, A, Baczko, K, ter Meulen, V & Billeter, MA 1987, 'Altered transcription of a defective measles virus genome derived from a diseased human brain.', EMBO Journal, vol. 6, no. 3, pp. 681-688.
Cattaneo R, Rebmann G, Schmid A, Baczko K, ter Meulen V, Billeter MA. Altered transcription of a defective measles virus genome derived from a diseased human brain. EMBO Journal. 1987 Mar;6(3):681-688.
Cattaneo, Roberto ; Rebmann, G. ; Schmid, A. ; Baczko, K. ; ter Meulen, V. ; Billeter, M. A. / Altered transcription of a defective measles virus genome derived from a diseased human brain. In: EMBO Journal. 1987 ; Vol. 6, No. 3. pp. 681-688.
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