Altered reactivity of tertiary mesenteric arteries following acute myocardial ischemia

Young Soo Han, Frank V. Brozovich

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: It is unknown if cardiac ischemia has any deleterious effect on the contractile properties of nonischemic, peripheral vascular beds. Thus, the objective of the present study was to determine whether acute myocardial ischemia results in peripheral vascular dysfunction. Methods and Results: This study characterized force maintenance and the sensitivity to acetylcholine (ACh)-mediated smooth muscle (SM) relaxation of tertiary (3rd) mesenteric arteries from Sprague-Dawley rats following 30 min of myocardial ischemia. Both the phosphorylation of nonmuscle (NM) light chain (LC) and SM-LCs as well as the expression of myosin phosphatase targeting subunit 1 (MYPT1) were also determined. Our data demonstrate that acute myocardial ischemia resulted in vascular dysfunction of 3rd mesenteric vessels, characterized by decreases in force maintenance, ACh-and cGMP-mediated SM relaxation, the phosphorylation of NM-LCs and SM-LCs, and MYPT1 expression. Ischemia was also associated with an increase in protein polyubiquitination, suggesting that during ischemia MYPT1 is targeted for degradation or proteolysis. Conclusion: Acute myocardial ischemia produces peripheral vascular dysfunction; the changes in LC phosphorylation and MYPT1 expression result in a decrease in both tone and the sensitivity to NO-mediated SM relaxation of the peripheral vasculature.

Original languageEnglish (US)
Pages (from-to)100-108
Number of pages9
JournalJournal of Vascular Research
Volume50
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Myosin-Light-Chain Phosphatase
Mesenteric Arteries
Muscle Relaxation
Myocardial Ischemia
Smooth Muscle
Blood Vessels
Ischemia
Phosphorylation
Acetylcholine
Maintenance
Smooth Muscle Myosins
Proteolysis
Sprague Dawley Rats
Light
Proteins

Keywords

  • 8-Bromo-cGMP
  • Acetylcholine
  • Mesenteric artery
  • Myosin phosphatase targeting subunit 1
  • Nonmuscle myosin

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Altered reactivity of tertiary mesenteric arteries following acute myocardial ischemia. / Han, Young Soo; Brozovich, Frank V.

In: Journal of Vascular Research, Vol. 50, No. 2, 02.2013, p. 100-108.

Research output: Contribution to journalArticle

Han, Young Soo ; Brozovich, Frank V. / Altered reactivity of tertiary mesenteric arteries following acute myocardial ischemia. In: Journal of Vascular Research. 2013 ; Vol. 50, No. 2. pp. 100-108.
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