Altered patterns of protein phosphorylation in articular chondrocytes treated with interleukin-1 or synovial cytokines

K. I. Hulkower, H. I. Georgescu, Christopher H Evans

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cultures of lapine articular chondrocytes were exposed to purified, human, recombinant interleukin-1 α or partially purified preparations of lapine, synovial, cytokines in the presence of [32P]orthophosphate. After 30 min incubation, phosphoproteins were extracted from the cells, separated by two-dimensional gel electrophoresis and visualized autoradiographically. Analysis of the autoradiograms revealed that interleukin-1 and the synovial factors produced marked changes in the pattern of protein phosphorylation. The synovial cytokines induced many of the same changes as interleukin-1, as well as a number of unique changes. This finding is consistent with the notion that, in addition to interleukin-1, synoviocytes secrete other cytokines which modulate the metabolism of chondrocytes. These data support the idea that signal transduction in chondrocytes responding to interleukin-1 involves the activation of one or more protein kinases.

Original languageEnglish (US)
Pages (from-to)228-232
Number of pages5
JournalFEBS Letters
Volume257
Issue number2
DOIs
StatePublished - Nov 6 1989
Externally publishedYes

Fingerprint

Phosphorylation
Chondrocytes
Interleukin-1
Joints
Cytokines
Proteins
Signal transduction
Phosphoproteins
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Metabolism
Protein Kinases
Signal Transduction
Gels
Chemical activation
Phosphates

Keywords

  • Chondrocyte-activating factor
  • Interleukin-1
  • Protein kinase
  • Protein phosphorylation
  • Signal transduction
  • Synovial cytokine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Altered patterns of protein phosphorylation in articular chondrocytes treated with interleukin-1 or synovial cytokines. / Hulkower, K. I.; Georgescu, H. I.; Evans, Christopher H.

In: FEBS Letters, Vol. 257, No. 2, 06.11.1989, p. 228-232.

Research output: Contribution to journalArticle

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