Alterations of nitric oxide synthase expression and activity during rat lung transplantation

Mingyao Liu, Lorraine Tremblay, Stephen D. Cassivi, Xiao Hui Bai, Eric Mourgeon, Andrew F. Pierre, Arthur S. Slutsky, Martin Post, Shaf Keshavjee

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Decreased nitric oxide (NO) production has been reported during lung transplantation in patients. To study the effects of ischemia and reperfusion on endogenous NO synthase (NOS) expression, both an ex vivo and an in vivo lung injury model for transplantation were used. Donor rat lungs were flushed with cold low-potassium dextran solution and subjected to either cold (4°C for 12 h) or warm (21°C for 4 h) ischemic preservation followed by reperfusion with an ex vivo model. A significant increase in inducible NOS and a decrease in endothelial NOS mRNA was found after reperfusion. These results were confirmed in a rat single-lung transplant model after warm preservation. Interestingly, protein contents of both inducible NOS and endothelial NOS increased in the transplanted lung after 2 h of reperfusion. However, the total activity of NOS in the transplanted lungs remained at very low levels. We conclude that ischemic lung preservation and reperfusion result in altered NOS gene and protein expression with inhibited NOS activity, which may contribute to the injury of lung transplants.

Original languageEnglish (US)
Pages (from-to)L1071-L1081
JournalAmerican Journal of Physiology
Volume278
Issue number5 PART 1
DOIs
StatePublished - May 2000
Externally publishedYes

Keywords

  • Acute lung injury
  • Early graft dysfunction
  • Ischemia-reperfusion

ASJC Scopus subject areas

  • Physiology (medical)

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  • Cite this

    Liu, M., Tremblay, L., Cassivi, S. D., Bai, X. H., Mourgeon, E., Pierre, A. F., Slutsky, A. S., Post, M., & Keshavjee, S. (2000). Alterations of nitric oxide synthase expression and activity during rat lung transplantation. American Journal of Physiology, 278(5 PART 1), L1071-L1081. https://doi.org/10.1152/ajplung.2000.278.5.l1071