Alterations in peripheral B cells and B cell progenitors following androgen ablation in mice

T. M. Ellis, M. T. Moser, P. T. Le, R. C. Flanigan, E. D. Kwon

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

The production of B lymphocytes is regulated in part by physiologic levels of androgens and estrogens. While these sex hormones down-regulate B lymphopoiesis, augmentation of B lymphopoiesis occurs under conditions where androgen or estrogen levels are decreased. In this study we examine the effect of androgen ablation of male mice on B lymphopoiesis and on the phenotypic composition of peripheral B lymphocyte populations. Spleen and thymic weights are significantly increased following castration, as is the total number of peripheral blood lymphocytes. However, the absolute numbers of B cells in the periphery are selectively increased following castration; the numbers of T cells, NK cells and granulocytes remain unchanged. The increase in circulating B cells is due largely to increases in the numbers of recent of recent bone marrow emigrants expressing a B220lo+CD24hl+ phenotype and these cells remain significantly elevated in castrated mice for up to 54 days post-castration. Similar increases in the percentages of newly emigrated B cells are observed in mice that lack a functional androgen receptor (Tfm). Finally, assessments of B cell progenitors in the bone marrow revealed significant increases in the relative numbers of IL-7 responsive B cell progenitors, including cells in Hardy fractions B (early pro-B cells), C (late pro-B cells), D (pre-B cells) and E (immature B cells). These findings demonstrate that androgen ablation following castration significantly and selectively alters the composition of peripheral B cells in mice. Further, these alterations result from the potentiating effects of androgen ablation on IL-7-responsive pro-B cell progenitors.

Original languageEnglish (US)
Pages (from-to)553-558
Number of pages6
JournalInternational Immunology
Volume13
Issue number4
DOIs
StatePublished - 2001

Keywords

  • Androgen ablation
  • B cell
  • B cell progenitor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Alterations in peripheral B cells and B cell progenitors following androgen ablation in mice'. Together they form a unique fingerprint.

Cite this