Alterations in liver chromatin during perinatal development of the rat

F. Chytil, S. R. Glasser, T. C. Spelsberg

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Chromatins were isolated from liver nuclei of 19-day fetuses, 2-, 5-, 21-day old and adult rats. Very little variation was observed in the mass ratio of total histones to DNA or in the spectrum of histones as determined by polyacrylamide gel electrophoresis. On the other hand, the amount and banding pattern of acidic proteins indicated pronounced changes during liver development. The composition of acidic proteins may be specific for the stage of development as evidenced immunochemically. Antibody against acidic protein-DNA complexes from adult rat liver were produced in rabbits. Whereas adult liver acidic protein-DNA complexes interacted strongly with the antibody, fetal liver preparations showed very little affinity. Complexes from 2-day-old animals reacted more strongly than fetal complexes while preparations from 5-day-old and 21-day-old displayed further increases in affinity. The results support the idea that chromatin acidic proteins play an important role in genetic expression during the ontogeny.

Original languageEnglish (US)
Pages (from-to)295-305
Number of pages11
JournalDevelopmental Biology
Volume37
Issue number2
DOIs
StatePublished - Apr 1974

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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