Alterations in insulin binding accompanying differentiation of 3T3-L1 preadipocytes

B. C. Reed, Scott H Kaufmann, J. C. Mackall, A. K. Student, M. D. Lane

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Abstract

Expression of the adipocyte phenotype by differentiating 3T3-LI preadipocytes occurs upon exposure of the cells to insulin. Differentiation-linked changes in 125I-labeled insulin binding to 3T3-LI cells were monitored and compared with those in nondifferentiating 3T3-C2 controls treated similarly. Without chronic insulin treatment, 3T3-LI cells failed to express the adipocyte phenotype but maintained a level of 25 000-35 000 insulin-binding sites per cell. Treatment of 3T3-LI cells with insulin resulted in an initial suppression of insulin binding followed by a 12-fold increase that paralleled the appearance of differentiated cells. A maximum of 170,000 insulin-binding sites per cell was attained for a population in which >75% of the cells had differentiated. The increase of insulin receptor level appears to be differentiation-dependent and is not a general response of cells to the culture conditions. 3T3-C2 cells maintained in the presence of insulin for 30 days exhibited the undifferentiated phenotype and suppressed levels of insulin binding (35,000 sites per cell). The binding capacity of 3T3-LI cells for epidermal growth factor remained unchanged between 25,000 and 40,000 sites per cell and was independent of the state of differentiation. Thus, induction by insulin of differentiation-linked expression in 3T3-LI cells results in receptor-specific changes. Insulin receptors increase in number but epidermal growth factor receptors remain constant.

Original languageEnglish (US)
Pages (from-to)4876-4880
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number11
StatePublished - 1977
Externally publishedYes

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3T3 Cells
Insulin
Insulin Receptor
Phenotype
Adipocytes
Binding Sites
Epidermal Growth Factor
Cell Culture Techniques
Population

ASJC Scopus subject areas

  • Genetics
  • General

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Alterations in insulin binding accompanying differentiation of 3T3-L1 preadipocytes. / Reed, B. C.; Kaufmann, Scott H; Mackall, J. C.; Student, A. K.; Lane, M. D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 74, No. 11, 1977, p. 4876-4880.

Research output: Contribution to journalArticle

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