TY - JOUR
T1 - Alteration of the nucleolar localization of poly(ADP-ribose) polymerase upon treatment with transcription inhibitors
AU - Desnoyers, Serge
AU - Kaufmann, Scott H.
AU - Poirier, Guy G.
N1 - Funding Information:
The authors are indebted to Dr. Georges Pelletier (CHUL Research Center, QueÂbec, Canada) for making the electron microscope available, Mr. Romano Puviani and Mrs. France Lepire for help in preparation of samples for electron microscopy and assistance with photography. This work was supported by Grant MT-6128 from the Canadian Medical Research Council. S.D. is supported by a postdoctoral Fellowship from ``le Fonds de la Recherhe en Sante du QueÂbec.'' S.H.K. is a Scholar of the Leukemia Society of America.
PY - 1996/8/25
Y1 - 1996/8/25
N2 - The effect of RNA, DNA, and protein synthesis inhibitors on the subnuclear localization of poly(ADP-ribose) polymerase (PARP) was examined. Indirect immunofluorescence indicated that PARP was distributed throughout the nuclei but concentrated in nucleoli of MDBK, HeLa, and CHO cells. Treatment with the DNA synthesis inhihitor cytosine arabinoside or the protein synthesis inhibitor cycloheximide did not change the distribution of PARP. In contrast, incubation with the RNA-synthesis inhibitor 5,6-dichloro- 1-β-ribofuranosyl-benzimidazole (DRB) caused PARP immunofluorescence to become evenly distributed throughout the nucleus. This phenomenon was observed after a 1-h incubation with a DRB concentration that inhibited [5,6- 3Hluridine incorporation by 75%. Similar results were obtained with actinomycin D. Immunoblotting showed that the DRB treatment did not cause any changes in the integrity and content of PARP. Removal of DRB from the media allowed PARP to reaccumulate in nucleoli within 1 h, suggesting that the nucleolar localization of PARP is dependent upon active RNA synthesis.
AB - The effect of RNA, DNA, and protein synthesis inhibitors on the subnuclear localization of poly(ADP-ribose) polymerase (PARP) was examined. Indirect immunofluorescence indicated that PARP was distributed throughout the nuclei but concentrated in nucleoli of MDBK, HeLa, and CHO cells. Treatment with the DNA synthesis inhihitor cytosine arabinoside or the protein synthesis inhibitor cycloheximide did not change the distribution of PARP. In contrast, incubation with the RNA-synthesis inhibitor 5,6-dichloro- 1-β-ribofuranosyl-benzimidazole (DRB) caused PARP immunofluorescence to become evenly distributed throughout the nucleus. This phenomenon was observed after a 1-h incubation with a DRB concentration that inhibited [5,6- 3Hluridine incorporation by 75%. Similar results were obtained with actinomycin D. Immunoblotting showed that the DRB treatment did not cause any changes in the integrity and content of PARP. Removal of DRB from the media allowed PARP to reaccumulate in nucleoli within 1 h, suggesting that the nucleolar localization of PARP is dependent upon active RNA synthesis.
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U2 - 10.1006/excr.1996.0259
DO - 10.1006/excr.1996.0259
M3 - Article
C2 - 8806461
AN - SCOPUS:0030601333
SN - 0014-4827
VL - 227
SP - 146
EP - 153
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -