Allergic inflammatory response to short ragweed allergenic extract in HLA-DQ transgenic mice lacking CD4 gene

S. P. Chapoval, K. Iijima, E. V. Marietta, M. K. Smart, A. I. Chapoval, Amy Andrews, C. S. David

Research output: Contribution to journalArticle

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Abstract

To investigate the role of HLA-DQ molecules and/or CD4+ T cells in the pathogenesis of allergic asthma, we generated HLA-DQ6 and HLA-DQ8 transgenic mice lacking endogenous class II (Aβnull) and CD4 genes and challenged them intranasally with short ragweed allergenic extract (SRW). We found that DQ6/CD4null mice developed a strong eosinophilic infiltration into the bronchoalveolar lavage and lung tissue, while DQ8/CD4null mice were normal. However, neither cytokines nor eosinophil peroxidase in the bronchoalveolar lavage of DQ6/CD4null mice was found. In addition, the airway reactivity to methacholine was elevated moderately in DQ6/CD4null mice compared with the high response in DQ/CD4+ counterparts and was only partially augmented by CD4+ T cell transfer. The DQ6/CD4null mice showed Th1/Th2-type cytokines and SRW-specific Abs in the immune sera in contrast to a direct Th2 response observed in DQ6/CD4+ mice. The proliferative response of spleen mononuclear cells and peribronchial lymph node cells demonstrated that the response to SRW in DQ6/CD4null mice was mediated by HLA-DQ-restricted CD4-CD8-NK1.1- T cells. FA CS analysis of PBMC and spleen mononuclear cells demonstrated an expansion of double-negative (DN) CD4-CD8-TCRαβ+ T cells in SRW-treated DQ6/CD4null mice. These cells produced IL-4, IL-5, IL-13, and IFN-γ, when stimulated with immobilized anti-CD3. IL-5 ELISPOT assay revealed that DN T cells were the cellular origin of IL-5 in allergen-challenged DQ6/CD4null mice. Our study shows a role for HLA-DQ-restricted CD4+ and DN T cells in the allergic response.

Original languageEnglish (US)
Pages (from-to)890-899
Number of pages10
JournalJournal of Immunology
Volume168
Issue number2
StatePublished - Jan 15 2002

Fingerprint

HLA-DQ Antigens
Ambrosia
Transgenic Mice
Genes
T-Lymphocytes
Interleukin-5
Bronchoalveolar Lavage
Spleen
Eosinophil Peroxidase
Cytokines
Enzyme-Linked Immunospot Assay
CD4 Antigens
Interleukin-13
Methacholine Chloride
Interleukin-4
Allergens
Immune Sera
Asthma
Lymph Nodes
Lung

ASJC Scopus subject areas

  • Immunology

Cite this

Chapoval, S. P., Iijima, K., Marietta, E. V., Smart, M. K., Chapoval, A. I., Andrews, A., & David, C. S. (2002). Allergic inflammatory response to short ragweed allergenic extract in HLA-DQ transgenic mice lacking CD4 gene. Journal of Immunology, 168(2), 890-899.

Allergic inflammatory response to short ragweed allergenic extract in HLA-DQ transgenic mice lacking CD4 gene. / Chapoval, S. P.; Iijima, K.; Marietta, E. V.; Smart, M. K.; Chapoval, A. I.; Andrews, Amy; David, C. S.

In: Journal of Immunology, Vol. 168, No. 2, 15.01.2002, p. 890-899.

Research output: Contribution to journalArticle

Chapoval, SP, Iijima, K, Marietta, EV, Smart, MK, Chapoval, AI, Andrews, A & David, CS 2002, 'Allergic inflammatory response to short ragweed allergenic extract in HLA-DQ transgenic mice lacking CD4 gene', Journal of Immunology, vol. 168, no. 2, pp. 890-899.
Chapoval SP, Iijima K, Marietta EV, Smart MK, Chapoval AI, Andrews A et al. Allergic inflammatory response to short ragweed allergenic extract in HLA-DQ transgenic mice lacking CD4 gene. Journal of Immunology. 2002 Jan 15;168(2):890-899.
Chapoval, S. P. ; Iijima, K. ; Marietta, E. V. ; Smart, M. K. ; Chapoval, A. I. ; Andrews, Amy ; David, C. S. / Allergic inflammatory response to short ragweed allergenic extract in HLA-DQ transgenic mice lacking CD4 gene. In: Journal of Immunology. 2002 ; Vol. 168, No. 2. pp. 890-899.
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